The effect of fluoroquinolones and antioxidans on biofilm formation by Proteus mirabilis strains.

Autor: Przekwas J; Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland., Gębalski J; Department of Pharmaceutical Botany and Pharmacognosy, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland., Kwiecińska-Piróg J; Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland., Wiktorczyk-Kapischke N; Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland., Wałecka-Zacharska E; Department of Food Hygiene and Consumer Health, Wrocław University of Environmental and Life Sciences, Wrocław, Poland., Gospodarek-Komkowska E; Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland., Rutkowska D; Medical Education Unit, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland., Skowron K; Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland. skowron238@wp.pl.
Jazyk: angličtina
Zdroj: Annals of clinical microbiology and antimicrobials [Ann Clin Microbiol Antimicrob] 2022 Jun 02; Vol. 21 (1), pp. 22. Date of Electronic Publication: 2022 Jun 02.
DOI: 10.1186/s12941-022-00515-5
Abstrakt: Background: Fluoroquinolones are a group of antibiotics used in urinary tract infections. Unfortunately, resistance to this group of drugs is currently growing. The combined action of fluoroquinolones and other antibacterial and anti-biofilm substances may extend the use of this therapeutic option by clinicians. The aim of the study was to determine the effect of selected fluoroquinolones and therapeutic concentrations of ascorbic acid and rutoside on biofilm formation by Proteus mirabilis.
Materials and Methods: The study included 15 strains of P. mirabilis isolated from urinary tract infections in patients of the University Hospital No. 1 dr A. Jurasz in Bydgoszcz (Poland). The metabolic activity of the biofilm treated with 0.4 mg/ml ascorbic acid, 0.02 µg/ml rutoside and chemotherapeutic agents (ciprofloxacin, norfloxacin) in the concentration range of 0.125-4.0 MIC (minimum inhibitory concentration) was assessed spectrophotometrically.
Results: Both ciprofloxacin and norfloxacin inhibited biofilm formation by the tested strains. The biofilm reduction rate was correlated with the increasing concentration of antibiotic used. No synergism in fluoroquinolones with ascorbic acid, rutoside or both was found. The ascorbic acid and rutoside combination, however, significantly decreased biofilm production.
Conclusions: Our research proves a beneficial impact of ascorbic acid with rutoside supplementation on biofilm of P. mirabilis strains causing urinary tract infections.
(© 2022. The Author(s).)
Databáze: MEDLINE
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