Detection of cell-cell interactions via photocatalytic cell tagging.

Autor: Oslund RC; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA. rob@induprolabs.com.; InduPro, Cambridge, MA, USA. rob@induprolabs.com., Reyes-Robles T; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA. tamara.reyes.robles@merck.com., White CH; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA., Tomlinson JH; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA., Crotty KA; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA., Bowman EP; Discovery Research, Merck & Co., Inc., San Francisco, CA, USA., Chang D; Genetics and Pharmacogenomics, Merck & Co., Inc., Boston, MA, USA., Peterson VM; Genetics and Pharmacogenomics, Merck & Co., Inc., Boston, MA, USA., Li L; Genetics and Pharmacogenomics, Merck & Co., Inc., Boston, MA, USA., Frutos S; SpliceBio, Barcelona, Spain., Vila-Perelló M; SpliceBio, Barcelona, Spain., Vlerick D; Ablynx, A Sanofi Company, Zwijnaarde, Belgium., Cromie K; Ablynx, A Sanofi Company, Zwijnaarde, Belgium., Perlman DH; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA., Ingale S; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA., Hara SDO; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA., Roberts LR; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA., Piizzi G; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA., Hett EC; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA., Hazuda DJ; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA.; Infectious Diseases and Vaccine Research, Merck & Co., Inc., West Point, PA, USA., Fadeyi OO; Merck Exploratory Science Center, Merck & Co., Inc., Cambridge, MA, USA. niyi@induprolabs.com.; InduPro, Cambridge, MA, USA. niyi@induprolabs.com.
Jazyk: angličtina
Zdroj: Nature chemical biology [Nat Chem Biol] 2022 Aug; Vol. 18 (8), pp. 850-858. Date of Electronic Publication: 2022 Jun 02.
DOI: 10.1038/s41589-022-01044-0
Abstrakt: The growing appreciation of immune cell-cell interactions within disease environments has led to extensive efforts to develop immunotherapies. However, characterizing complex cell-cell interfaces in high resolution remains challenging. Thus, technologies leveraging therapeutic-based modalities to profile intercellular environments offer opportunities to study cell-cell interactions with molecular-level insight. We introduce photocatalytic cell tagging (PhoTag) for interrogating cell-cell interactions using single-domain antibodies (VHHs) conjugated to photoactivatable flavin-based cofactors. Following irradiation with visible light, the flavin photocatalyst generates phenoxy radical tags for targeted labeling. Using this technology, we demonstrate selective synaptic labeling across the PD-1/PD-L1 axis in antigen-presenting cell-T cell systems. In combination with multiomics single-cell sequencing, we monitored interactions between peripheral blood mononuclear cells and Raji PD-L1 B cells, revealing differences in transient interactions with specific T cell subtypes. The utility of PhoTag in capturing cell-cell interactions will enable detailed profiling of intercellular communication across different biological systems.
(© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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