Responsive Nanogel Probe for Ratiometric Fluorescent Sensing of pH and Strain in Hydrogels.

Autor: Zhu M; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K., Lu D; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K., Wu S; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K., Lian Q; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K., Wang W; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K., Milani AH; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K., Cui Z; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K., Nguyen NT; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K., Chen M; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K., Lyon LA; Schmid College of Science and Technology, Chapman University, Orange, California 92866, United States., Adlam DJ; Division of Cell Matrix Biology and Regenerative Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester M13 9PT, U.K., Freemont AJ; Division of Cell Matrix Biology and Regenerative Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester M13 9PT, U.K.; NIHR Manchester Musculoskeletal Biomedical Research Unit, Central Manchester Foundation Trust, Manchester Academic Health Science Centre, Manchester, U.K., Hoyland JA; Division of Cell Matrix Biology and Regenerative Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester M13 9PT, U.K.; NIHR Manchester Musculoskeletal Biomedical Research Unit, Central Manchester Foundation Trust, Manchester Academic Health Science Centre, Manchester, U.K., Saunders BR; School of Materials, University of Manchester, MSS Tower, Manchester M13 9PL, U.K.
Jazyk: angličtina
Zdroj: ACS macro letters [ACS Macro Lett] 2017 Nov 21; Vol. 6 (11), pp. 1245-1250. Date of Electronic Publication: 2017 Oct 25.
DOI: 10.1021/acsmacrolett.7b00709
Abstrakt: In this study a new pH-responsive nanogel probe containing a complementary nonradiative resonance energy transfer (NRET) fluorophore pair is investigated and its ability to act as a versatile probe of network-related changes in three hydrogels demonstrated. Fluorescent sensing using NRET is a powerful method for studying relationships between Angstrom length-scale structure and macroscopic properties of soft matter. Unfortunately, inclusion of NRET fluorophores into such materials requires material-specific chemistry. Here, low concentrations of preformed nanogel probes were included into hydrogel hosts. Ratiometric photoluminescence (PL) data for the gels labeled with the nanogel probes enabled pH-triggered swelling and deswelling to be studied as well as Ca 2+ -triggered collapse and solute release. PL measurements during compression of a nanogel probe-labeled nanocomposite gel demonstrated mechanochromic behavior and strain sensing. The new nanogel probes have excellent potential for investigating the internal structures of gels and provide a versatile ratiometric fluorescent platform for studying pH and strain.
Databáze: MEDLINE