Leaf transcriptome profiling of contrasting sugarcane genotypes for drought tolerance under field conditions.

Autor: Contiliani DF; Instituto Agronômico (IAC), Centro de Cana, Ribeirão Preto, Brazil.; Programa de Pós-Graduação em Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil., de Oliveira Nebó JFC; Centro de Energia Nuclear na Agricultura (CENA), Universidade de São Paulo, Piracicaba, Brazil., Ribeiro RV; Laboratory of Crop Physiology, Department of Plant Biology, Institute of Biology, University of Campinas, Campinas, Brazil., Andrade LM; Instituto Agronômico (IAC), Centro de Cana, Ribeirão Preto, Brazil., Peixoto Júnior RF; Instituto Agronômico (IAC), Centro de Cana, Ribeirão Preto, Brazil., Lembke CG; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil., Machado RS; Instituto Agronômico (IAC), Centro de Ecofisiologia e Biofísica, Campinas, Brazil., Silva DN; Instituto Agronômico (IAC), Centro de Cana, Ribeirão Preto, Brazil., Belloti M; Centro de Energia Nuclear na Agricultura (CENA), Universidade de São Paulo, Piracicaba, Brazil., de Souza GM; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil., Perecin D; Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, Brazil., Pereira TC; Programa de Pós-Graduação em Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.; Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., de Matos Pires RC; Instituto Agronômico (IAC), Centro de Ecofisiologia e Biofísica, Campinas, Brazil., Fontoura PR; Jalles Machado Sugar Mill, Goianesia, Brazil., Landell MGA; Instituto Agronômico (IAC), Centro de Cana, Ribeirão Preto, Brazil., Figueira A; Centro de Energia Nuclear na Agricultura (CENA), Universidade de São Paulo, Piracicaba, Brazil., Creste S; Instituto Agronômico (IAC), Centro de Cana, Ribeirão Preto, Brazil. silvana.creste@sp.gov.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jun 01; Vol. 12 (1), pp. 9153. Date of Electronic Publication: 2022 Jun 01.
DOI: 10.1038/s41598-022-13158-5
Abstrakt: Drought is the most detrimental abiotic stress to sugarcane production. Nevertheless, transcriptomic analyses remain scarce for field-grown plants. Here we performed comparative transcriptional profiling of two contrasting sugarcane genotypes, 'IACSP97-7065' (drought-sensitive) and 'IACSP94-2094' (drought-tolerant) grown in a drought-prone environment. Physiological parameters and expression profiles were analyzed at 42 (May) and 117 (August) days after the last rainfall. The first sampling was done under mild drought (soil water potential of -60 kPa), while the second one was under severe drought (soil water potential of -75 kPa). Microarray analysis revealed a total of 622 differentially expressed genes in both sugarcane genotypes under mild and severe drought stress, uncovering about 250 exclusive transcripts to 'IACSP94-2094' involved in oxidoreductase activity, transcriptional regulation, metabolism of amino acids, and translation. Interestingly, the enhanced antioxidant system of 'IACSP94-2094' may protect photosystem II from oxidative damage, which partially ensures stable photochemical activity even after 117 days of water shortage. Moreover, the tolerant genotype shows a more extensive set of responsive transcription factors, promoting the fine-tuning of drought-related molecular pathways. These results help elucidate the intrinsic molecular mechanisms of a drought-tolerant sugarcane genotype to cope with ever-changing environments, including prolonged water deficit, and may be useful for plant breeding programs.
(© 2022. The Author(s).)
Databáze: MEDLINE
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