Enhancing the function of PLGA-collagen scaffold by incorporating TGF-β1-loaded PLGA-PEG-PLGA nanoparticles for cartilage tissue engineering using human dental pulp stem cells.

Autor: Ghandforoushan P; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran., Hanaee J; Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.; Pharmaceutical Analysis Research Center, Tabriz University of Medicinal Science, Tabriz, Iran., Aghazadeh Z; Stem Cell Research Center, Oral Medicine Department, Tabriz University of Medical Sciences, Tabriz, Iran., Samiei M; Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran., Navali AM; Department of Orthopedy, Tabriz University of Medical Sciences, Tabriz, Iran., Khatibi A; Department of Biotechnology, Alzahra University, Tehran, Iran., Davaran S; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. davaran@tbzmed.ac.ir.; Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. davaran@tbzmed.ac.ir.; Applied Drug Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. davaran@tbzmed.ac.ir.
Jazyk: angličtina
Zdroj: Drug delivery and translational research [Drug Deliv Transl Res] 2022 Dec; Vol. 12 (12), pp. 2960-2978. Date of Electronic Publication: 2022 Jun 01.
DOI: 10.1007/s13346-022-01161-2
Abstrakt: Since cartilage has a limited capacity for self-regeneration, treating cartilage degenerative disorders is a long-standing difficulty in orthopedic medicine. Researchers have scrutinized cartilage tissue regeneration to handle the deficiency of cartilage restoration capacity. This investigation proposed to compose an innovative nanocomposite biomaterial that enhances growth factor delivery to the injured cartilage site. Here, we describe the design and development of the biocompatible poly(lactide-co-glycolide) acid-collagen/poly(lactide-co-glycolide)-poly(ethylene glycol)-poly(lactide-co-glycolide) (PLGA-collagen/PLGA-PEG-PLGA) nanocomposite hydrogel containing transforming growth factor-β1 (TGF-β1). PLGA-PEG-PLGA nanoparticles were employed as a delivery system embedding TGF-β1 as an articular cartilage repair therapeutic agent. This study evaluates various physicochemical aspects of fabricated scaffolds by 1 HNMR, FT-IR, SEM, BET, and DLS methods. The physicochemical features of the developed scaffolds, including porosity, density, degradation, swelling ratio, mechanical properties, morphologies, BET, ELISA, and cytotoxicity were assessed. The cell viability was investigated with the MTT test. Chondrogenic differentiation was assessed via Alcian blue staining and RT-PCR. In real-time PCR testing, the expression of Sox-9, collagen type II, and aggrecan genes was monitored. According to the results, human dental pulp stem cells (hDPSCs) exhibited high adhesion, proliferation, and differentiation on PLGA-collagen/PLGA-PEG-PLGA-TGFβ1 nanocomposite scaffolds compared to the control groups. SEM images displayed suitable cell adhesion and distribution of hDPSCs throughout the scaffolds. RT-PCR assay data displayed that TGF-β1 loaded PLGA-PEG-PLGA nanoparticles puts forward chondroblast differentiation in hDPSCs through the expression of chondrogenic genes. The findings revealed that PLGA-collagen/PLGA-PEG-PLGA-TGF-β1 nanocomposite hydrogel can be utilized as a supportive platform to support hDPSCs differentiation by implementing specific physio-chemical features.
(© 2022. Controlled Release Society.)
Databáze: MEDLINE
Externí odkaz: