| Autor: |
Marin IA; Department of Biology, Stanford University, Stanford, CA 94035.; Department of Neurobiology, Stanford University, Stanford, CA 94035., Gutman-Wei AY; Department of Biology, Stanford University, Stanford, CA 94035.; Department of Neurobiology, Stanford University, Stanford, CA 94035., Chew KS; Department of Biology, Stanford University, Stanford, CA 94035.; Department of Neurobiology, Stanford University, Stanford, CA 94035., Raissi AJ; Department of Biology, Stanford University, Stanford, CA 94035.; Department of Neurobiology, Stanford University, Stanford, CA 94035., Djurisic M; Department of Biology, Stanford University, Stanford, CA 94035.; Department of Neurobiology, Stanford University, Stanford, CA 94035., Shatz CJ; Department of Biology, Stanford University, Stanford, CA 94035.; Department of Neurobiology, Stanford University, Stanford, CA 94035. |
| Abstrakt: |
During developmental critical periods, circuits are sculpted by a process of activity-dependent competition. The molecular machinery involved in regulating the complex process of responding to different levels of activity is now beginning to be identified. Here, we show that the nonclassical major histocompatibility class I (MHCI) molecule Qa-1 is expressed in the healthy brain in layer 6 corticothalamic neurons. In the visual cortex, Qa-1 expression begins during the critical period for ocular dominance (OD) plasticity and is regulated by neuronal activity, suggesting a role in regulating activity-dependent competition. Indeed, in mice lacking Qa-1, OD plasticity is perturbed. Moreover, signaling through CD94/NKG2, a known cognate Qa-1 heterodimeric receptor in the immune system, is implicated: selectively targeting this interaction phenocopies the plasticity perturbation observed in Qa-1 knockouts. In the cortex, CD94/NKG2 is expressed by microglial cells, which undergo activity-dependent changes in their morphology in a Qa-1–dependent manner. Our study thus reveals a neuron–microglial interaction dependent upon a nonclassical MHCI molecule expressed in L6 neurons, which regulates plasticity in the visual cortex. These results also point to an unexpected function for the Qa-1/HLA-E (ligand) and CD94/NKG2 (receptor) interaction in the nervous system, in addition to that described in the immune system. |
