Development and Characterization of a Nonelectronic Versatile Oxygenating Perfusion System for Tissue Preservation.

Autor: Portillo DJ; Department of Mechanical Engineering, The University of Texas at San Antonio, 1 UTSA Circle, San Antonio, TX, 78249, USA., Gonzalez J; Department of Mechanical Engineering, The University of Texas at San Antonio, 1 UTSA Circle, San Antonio, TX, 78249, USA., Villarreal C; Department of Mechanical Engineering, The University of Texas at San Antonio, 1 UTSA Circle, San Antonio, TX, 78249, USA., Salazar SJ; Department of Mechanical Engineering, The University of Texas at San Antonio, 1 UTSA Circle, San Antonio, TX, 78249, USA., Fasci A; Department of Mechanical Engineering, The University of Texas at San Antonio, 1 UTSA Circle, San Antonio, TX, 78249, USA., Wearden B; Department of Mechanical Engineering, The University of Texas at San Antonio, 1 UTSA Circle, San Antonio, TX, 78249, USA., Oseghale J; Department of Biomedical Engineering, The University of Texas at San Antonio, San Antonio, USA., Khalil A; Department of Mechanical Engineering, The University of Texas at San Antonio, 1 UTSA Circle, San Antonio, TX, 78249, USA., Perillo T; Department of Mechanical Engineering, The University of Texas at San Antonio, 1 UTSA Circle, San Antonio, TX, 78249, USA., Muenchow L; Department of Biology, The University of Texas at San Antonio, San Antonio, USA., De Lorenzo R; Department of Emergency Medicine, UT Health San Antonio, San Antonio, USA., Hood RL; Department of Mechanical Engineering, The University of Texas at San Antonio, 1 UTSA Circle, San Antonio, TX, 78249, USA. lyle.hood@utsa.edu.; Department of Biomedical Engineering, The University of Texas at San Antonio, San Antonio, USA. lyle.hood@utsa.edu.; Department of Emergency Medicine, UT Health San Antonio, San Antonio, USA. lyle.hood@utsa.edu.
Jazyk: angličtina
Zdroj: Annals of biomedical engineering [Ann Biomed Eng] 2022 Aug; Vol. 50 (8), pp. 978-990. Date of Electronic Publication: 2022 Jun 01.
DOI: 10.1007/s10439-022-02977-2
Abstrakt: Oxygenated machine perfusion of human organs has been shown to improve both preservation quality and time duration when compared to the current gold standard: static cold storage. However, existing machine perfusion devices designed for preservation and transportation of transplantable organs are too complicated and organ-specific to merit use as a solution for all organs. This work presents a novel, portable, and nonelectronic device potentially capable of delivering oxygenated machine perfusion to a variety of organs. An innovative pneumatic circuit system regulates a compressed oxygen source that cyclically inflates and deflates silicone tubes, which function as both the oxygenator and perfusion pump. Different combinations of silicone tubes in single or parallel configurations, with lengths ranging from 1.5 to 15.2 m, were evaluated at varying oxygen pressures from 27.6 to 110.3 kPa. The silicone tubes in parallel configurations produced higher peak perfusion pressures (70% increase), mean flow rates (102% increase), and oxygenation rates (268% increase) than the single silicone tubes that had equivalent total lengths. While pumping against a vascular resistance element that mimicked a kidney, the device achieved perfusion pressures (8.4-131.6 mmHg), flow rates (2.0-40.2 mL min -1 ), and oxygenation rates (up to 388 μmol min -1 ) that are consistent with values used in previous kidney preservation studies. The nonelectronic device achieved those perfusion parameters using 4.4 L min -1 of oxygen to operate. These results demonstrate that oxygenated machine perfusion can be successfully achieved without any electronic components.
(© 2022. The Author(s) under exclusive licence to Biomedical Engineering Society.)
Databáze: MEDLINE
Externí odkaz: