Correlation of quantitative polymerase chain reaction with clinical characteristics of patients with viral retinitis.

Autor: Mohan S; Department of Uvea, SankaraNethralaya, Medical Research Foundation, Chennai, Tamil Nadu, India., Dhanurekha L; Sankara Nethralaya Referral Laboratory, Medical Research Foundation, Chennai, Tamil Nadu, India., Janani MK; Sankara Nethralaya Referral Laboratory, Medical Research Foundation, Chennai, Tamil Nadu, India., Biswas J; Department of Uvea, SankaraNethralaya, Medical Research Foundation, Chennai, Tamil Nadu, India.
Jazyk: angličtina
Zdroj: Indian journal of ophthalmology [Indian J Ophthalmol] 2022 Jun; Vol. 70 (6), pp. 2090-2093.
DOI: 10.4103/ijo.IJO_3000_21
Abstrakt: Purpose: To evaluate the correlation of quantitative real-time polymerase chain reaction (qRT-PCR) to the clinical characteristics of patients with viral retinitis.
Methods: Retrospective case series.
Results: Aqueous or vitreous samples of 20 out of 35 eyes showed qRT-PCR positivity for virus etiology (57.14%). Cytomegalovirus (CMV) was most commonly identified in nine eyes (45%). The mean DNA copy number was 2,68,339.65 copies/mL (range: 90-3205397). DNA copy number significantly correlated with the extent of clinical involvement (P = 0.013); however, there was no correlation between DNA copy number and presenting visual acuity (P = 0.31), macular involvement (P = 0.675), optic nerve involvement (P = 0.14), and development of retinal detachment (P = 0.73). There was a significant correlation between the number of DNA copies and the timing of sampling (P = 0.0005). Samples taken earlier in the course of the disease had higher viral copies than later ones.
Conclusion: qRT-PCR is useful in confirming a viral etiology in over 50% of cases of suspected viral retinitis. It correlates well with the extent of clinical involvement and timing of sampling.
Competing Interests: None
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje