Long Lasting Antibodies From Convalescent Pertussis Patients Induce ROS Production and Bacterial Killing by Human Neutrophils.

Autor:	Kroes MM; Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, Netherlands.; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., van Vliet LC; Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, Netherlands., Jacobi RHJ; Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, Netherlands., Kuipers B; Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, Netherlands., Pieren DKJ; Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, Netherlands., Miranda-Bedate A; Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, Netherlands., van Els CACM; Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, Netherlands.; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., Pinelli E; Center for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, Netherlands.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 May 12; Vol. 12, pp. 888412. Date of Electronic Publication: 2022 May 12 (Print Publication: 2022).
DOI:	10.3389/fcimb.2022.888412
Abstrakt:	Pertussis is a respiratory infection caused by the Gram-negative bacterium Bordetella pertussis . Despite high vaccination coverage this disease remains a public health concern worldwide. A better understanding of the protective immune responses to B. pertussis is required for the development of improved vaccines. The aim of this study was to determine the production of reactive oxygen species (ROS) by human neutrophils in response to B. pertussis and to determine the contribution of opsonizing antibodies from convalescent pertussis patients in this response. The serum samples from convalescent patients were taken at <3, 9, 18 and 36 months after diagnosis of pertussis. Also included were sera from healthy age-matched controls. We show that neutrophils produced high levels of ROS in response to opsonized, compared to non-opsonized, B. pertussis and that this effect was independent of the time the convalescent serum samples were taken. This indicates the presence of functional opsonizing antibodies up to 3 years after B. pertussis infection. While opsonization of B. pertussis with serum samples from uninfected controls also induced ROS production, sera from infected individuals induced significantly higher ROS levels. Spearman correlations analysis showed that IgG antibodies targeting fimbriae3 followed by pertactin, and BrkA correlate with ROS production. Additionally, we observed that neutrophils killed opsonized B. pertussis in a ROS-dependent manner. Searching for other antigen-specific antibodies from convalescent pertussis patients involved in ROS production by neutrophils may assist in the identification of novel antigens to improve the current pertussis vaccines. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Kroes, van Vliet, Jacobi, Kuipers, Pieren, Miranda-Bedate, van Els and Pinelli.)
Databáze:	MEDLINE
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