Histological and Top-Down Proteomic Analyses of the Visual Pathway in the Cuprizone Demyelination Model.

Autor: Almuslehi MSM; Department of Physiology & Pharmacology, College of Veterinary Medicine, University of Diyala, Baqubah, Diyala, 32001, Iraq.; School of Medicine, Western Sydney University, Penrith, NSW, 2751, Australia., Sen MK; School of Medicine, Western Sydney University, Penrith, NSW, 2751, Australia.; Charles Perkins Centre, School of Medical Sciences, University of Sydney, Camperdown, NSW, 2006, Sydney, Australia., Shortland PJ; School of Science, Western Sydney University, Penrith, NSW, 2751, Australia., Mahns DA; School of Medicine, Western Sydney University, Penrith, NSW, 2751, Australia. d.mahns@westernsydney.edu.au.; Department of Integrative Physiology, School of Medicine, Western Sydney University, Locked Bag 1797, Penrith, NSW 2571, Australia. d.mahns@westernsydney.edu.au., Coorssen JR; Department of Health Sciences, Faculty of Applied Health Sciences, and Department of Biological Sciences, Faculty of Mathematics and Science, Brock University, ON, L2S 3A1, St. Catharine's, Canada. jcoorssen@brocku.ca.
Jazyk: angličtina
Zdroj: Journal of molecular neuroscience : MN [J Mol Neurosci] 2022 Jun; Vol. 72 (6), pp. 1374-1401. Date of Electronic Publication: 2022 May 30.
DOI: 10.1007/s12031-022-01997-w
Abstrakt: A change in visual perception is a frequent early symptom of multiple sclerosis (MS), the pathoaetiology of which remains unclear. Following a slow demyelination process caused by 12 weeks of low-dose (0.1%) cuprizone (CPZ) consumption, histology and proteomics were used to investigate components of the visual pathway in young adult mice. Histological investigation did not identify demyelination or gliosis in the optic tracts, pretectal nuclei, superior colliculi, lateral geniculate nuclei or visual cortices. However, top-down proteomic assessment of the optic nerve/tract revealed a significant change in the abundance of 34 spots in high-resolution two-dimensional (2D) gels. Subsequent liquid chromatography-tandem mass spectrometry (LC-TMS) analysis identified alterations in 75 proteoforms. Literature mining revealed the relevance of these proteoforms in terms of proteins previously implicated in animal models, eye diseases and human MS. Importantly, 24 proteoforms were not previously described in any animal models of MS, eye diseases or MS itself. Bioinformatic analysis indicated involvement of these proteoforms in cytoskeleton organization, metabolic dysregulation, protein aggregation and axonal support. Collectively, these results indicate that continuous CPZ-feeding, which evokes a slow demyelination, results in proteomic changes that precede any clear histological changes in the visual pathway and that these proteoforms may be potential early markers of degenerative demyelinating conditions.
(© 2022. Crown.)
Databáze: MEDLINE
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