Longitudinal Evaluation of Gut Bacteriomes and Viromes after Fecal Microbiota Transplantation for Eradication of Carbapenem-Resistant Enterobacteriaceae .

Autor:	Liu Q; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Zuo T; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Lu W; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Yeoh YK; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Microbiology, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Su Q; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Xu Z; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Tang W; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Yang K; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Zhang F; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Lau LHS; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Lui RNS; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Chin ML; Department of Microbiology, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Wong R; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Cheung CP; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Zhu W; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Chan PKS; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Microbiology, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Chan FKL; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Lui GC; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China., Ng SC; Center for Gut Microbiota Research, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Department of Medicine and Therapeutics, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.; Microbiota I-Center, Hong Kong, China.; Institute of Digestive Disease, State Key Laboratory of Digestive Disease, LKS Institute of Health Sciences, The Chinese University of Hong Konggrid.10784.3a, Hong Kong, China.
Jazyk:	angličtina
Zdroj:	MSystems [mSystems] 2022 Jun 28; Vol. 7 (3), pp. e0151021. Date of Electronic Publication: 2022 Jun 01.
DOI:	10.1128/msystems.01510-21
Abstrakt:	Understanding the role of fecal microbiota transplantation (FMT) in the decolonization of multidrug-resistant organisms (MDRO) is critical. Specifically, little is known about virome changes in MDRO-infected subjects treated with FMT. Using shotgun metagenomic sequencing, we characterized longitudinal dynamics of the gut virome and bacteriome in three recipients who successfully decolonized carbapenem-resistant Enterobacteriaceae (CRE), including Klebsiella spp. and Escherichia coli, after FMT. We observed large shifts of the fecal bacterial microbiota resembling a donor-like community after transfer of a fecal microbiota dominated by the genus Ruminococcus . We found a substantial expansion of Klebsiella phages after FMT with a concordant decrease of Klebsiella spp. and striking increase of Escherichia phages in CRE E. coli carriers after FMT. We also observed the CRE elimination and similar evolution of Klebsiella phage in mice, which may play a role in the collapse of the Klebsiella population after FMT. In summary, our pilot study documented bacteriome and virome alterations after FMT which mediate many of the effects of FMT on the gut microbiome community. IMPORTANCE Fecal microbiota transplantation (FMT) is an effective treatment for multidrug-resistant organisms; however, introducing a complex mixture of microbes also has unknown consequences for landscape features of gut microbiome. We sought to understand bacteriome and virome alterations in patients undergoing FMT to treat infection with carbapenem-resistant Enterobacteriaceae . This finding indicates that transkingdom interactions between the virome and bacteriome communities may have evolved in part to support efficient FMT for treating CRE.
Databáze:	MEDLINE
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