Development of Breeder-Friendly KASP Markers from Genome-Wide Association Studies Results.

Autor:	Makhoul M; Department of Plant Breeding, Justus Liebig University, Giessen, Germany., Obermeier C; Department of Plant Breeding, Justus Liebig University, Giessen, Germany. christian.obermeier@agrar.uni-giessen.de.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2481, pp. 287-310.
DOI:	10.1007/978-1-0716-2237-7_17
Abstrakt:	Array-based SNP markers are commonly used in genome-wide association studies (GWAS) to identify genomic regions involved in important agronomical traits. However, conversion of these SNP markers into breeder-friendly kompetitive allele-specific PCR (KASP) markers for use in marker-assisted selection is often challenging. In this chapter we describe general considerations and successfully applied protocols for the conversion of Illumina array SNP markers into locus-specific KASP markers with a special emphasis and examples on how to overcome difficulties in polyploid wheat. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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