Development of Breeder-Friendly KASP Markers from Genome-Wide Association Studies Results.
Autor: | Makhoul M; Department of Plant Breeding, Justus Liebig University, Giessen, Germany., Obermeier C; Department of Plant Breeding, Justus Liebig University, Giessen, Germany. christian.obermeier@agrar.uni-giessen.de. |
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Jazyk: | angličtina |
Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2481, pp. 287-310. |
DOI: | 10.1007/978-1-0716-2237-7_17 |
Abstrakt: | Array-based SNP markers are commonly used in genome-wide association studies (GWAS) to identify genomic regions involved in important agronomical traits. However, conversion of these SNP markers into breeder-friendly kompetitive allele-specific PCR (KASP) markers for use in marker-assisted selection is often challenging. In this chapter we describe general considerations and successfully applied protocols for the conversion of Illumina array SNP markers into locus-specific KASP markers with a special emphasis and examples on how to overcome difficulties in polyploid wheat. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
Databáze: | MEDLINE |
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