Circulating hyaluronan as a marker of endothelial glycocalyx damage in dogs with myxomatous mitral valve disease and dogs in a hypercoagulable state.

Autor: Lawrence-Mills SJ; Bristol Renal, Bristol Medical School, University of Bristol, Bristol, UK; The Royal Veterinary College, University of London, North Mymms, UK. Electronic address: slawrencemills21@rvc.ac.uk., Hezzell MJ; Bristol Veterinary School, University of Bristol, Langford, UK., Adamantos SE; Langford Vets, Langford House, Langford, UK; Paragon Veterinary Referrals, Wakefield, UK., Chan I; Bristol Veterinary School, University of Bristol, Langford, UK., Borgeat K; Langford Vets, Langford House, Langford, UK., Rose Payne J; Langford Vets, Langford House, Langford, UK., Satchell S; Bristol Renal, Bristol Medical School, University of Bristol, Bristol, UK., Welsh GI; Bristol Renal, Bristol Medical School, University of Bristol, Bristol, UK., Foster RR; Bristol Renal, Bristol Medical School, University of Bristol, Bristol, UK., Finch N; Bristol Renal, Bristol Medical School, University of Bristol, Bristol, UK; Langford Vets, Langford House, Langford, UK.
Jazyk: angličtina
Zdroj: Veterinary journal (London, England : 1997) [Vet J] 2022 Jul; Vol. 285, pp. 105845. Date of Electronic Publication: 2022 May 28.
DOI: 10.1016/j.tvjl.2022.105845
Abstrakt: The endothelial glycocalyx (eGlx) lines the luminal surface of endothelial cells, maintaining vascular health. Glycocalyx damage is pathophysiologically important in many diseases across species however few studies have investigated its breakdown in naturally occurring disease in dogs. The aims of the study were to investigate eGlx damage in dogs with myxomatous mitral valve disease (MMVD) diagnosed on echocardiography, and dogs in a hypercoagulable state diagnosed using thromboelastography (TEG), by measuring serum hyaluronan concentrations. Serum hyaluronan was quantified in dogs with MMVD (n = 27), hypercoagulability (n = 21), and in healthy controls dogs (n = 18). Serum hyaluronan concentrations were measured using a commercially-available ELISA validated for use in dogs. Hyaluronan concentrations were compared among groups using Kruskal-Wallis tests, and post-hoc with Dunn's tests. Serum hyaluronan concentrations (median [range]) were significantly increased in dogs with MMVD (62.4 [22.8-201] ng/mL; P = 0.031) and hypercoagulability (92.40 [16.9-247.6] ng/mL; P < 0.001) compared to controls (45.7 [8.7-80.2] ng/mL). Measurement of serum hyaluronan concentration offers a clinically applicable marker of eGlx health and suggests the presence of eGlx damage in dogs with MMVD and dogs in a hypercoagulable state.
(Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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