One-step sustainable extraction of Silymarin compounds of wild Algerian milk thistle (Silybum marianum) seeds using Gas Expanded Liquids.

Autor: Abderrezag N; Laboratory of Environmental Processes Engineering, University of Salah Boubnider Constantine 3, Ali Mendjli, 25000 Constantine, Algeria; Profesora Facultad de Ingeniería Agroindustrial, Universidad de Nariño (UdeNar), Pasto, Colombia., Montenegro ZJS; Profesora Facultad de Ingeniería Agroindustrial, Universidad de Nariño (UdeNar), Pasto, Colombia., Louaer O; Laboratory of Environmental Processes Engineering, University of Salah Boubnider Constantine 3, Ali Mendjli, 25000 Constantine, Algeria., Meniai AH; Laboratory of Environmental Processes Engineering, University of Salah Boubnider Constantine 3, Ali Mendjli, 25000 Constantine, Algeria., Cifuentes A; Foodomics Laboratory, Bioactivity and Food Analysis Department, Institute of Food Science Research CIAL (CSIC-UAM), Nicolas Cabrera 9, 28049 Madrid, Spain., Ibáñez E; Foodomics Laboratory, Bioactivity and Food Analysis Department, Institute of Food Science Research CIAL (CSIC-UAM), Nicolas Cabrera 9, 28049 Madrid, Spain., Mendiola JA; Foodomics Laboratory, Bioactivity and Food Analysis Department, Institute of Food Science Research CIAL (CSIC-UAM), Nicolas Cabrera 9, 28049 Madrid, Spain. Electronic address: j.mendiola@csic.es.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2022 Jul 19; Vol. 1675, pp. 463147. Date of Electronic Publication: 2022 May 18.
DOI: 10.1016/j.chroma.2022.463147
Abstrakt: This work reports the application of Gas Expanded Liquid (GXL) extraction to concentrate the flavonolignan fraction (silymarin) and taxifolin from Silybum marianum seeds, which have proven to be highly valuable health-promoting compounds. GXL using green solvents was used to isolate silymarin with the objective of replacing conventional methods. In one hand, the effect of different compositions of solvents, aqueous ethanol (20%, 50% or 80% (v/v)) at different CO 2 /liquid (25, 50 and 75%) ratios, on the GXL extraction was investigated. The obtained extracts have been chemically and functionally characterized by means of UHPLC-ESI-MS/MS (triple quadrupole) and in-vitro assays such as anti-inflammatory, anti-cholinergic and antioxidant. Results revealed that the operating conditions influenced the extraction yield, the total phenolic content and the presence of the target compounds. The best obtained yield was 55.97% using a ternary mixture of solvents composed of CO 2 :EtOH:H 2 O (25:60:15) at 40 °C and 9 MPa in 160 min. Furthermore, the results showed that obtained extracts had significant antioxidant and anti-inflammatory activities (with best IC 50 value of 8.80 µg/mL and 28.52 µg/mL, respectively) but a moderate anti-cholinesterase activity (with best IC 50 value of 125.09 µg/mL). Otherwise, the concentration of silymarin compounds in extract can go up to 59.6% using the present one-step extraction method without further purification, being silybin A+B the predominant identified compound, achieving value of 545.73 (mg silymarin/g of extract). The obtained results demonstrate the exceptional potential of GXL to extract high-added values molecules under sustainable conditions from different matrices.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: