Biotechnological production and application of epsilon-poly-L-lysine (ε-PL): biosynthesis and its metabolic regulation.

Autor: Wang D; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China. wangdahong2003@163.com.; Henan Engineering Research Center of Food Microbiology, Luoyang, 471023, China. wangdahong2003@163.com., Wang H; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China., Wu J; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China., Hou Y; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China., Sun J; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China.; Henan Engineering Research Center of Food Microbiology, Luoyang, 471023, China., Yuan J; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China., Gu S; College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China.; Henan Engineering Research Center of Food Microbiology, Luoyang, 471023, China.
Jazyk: angličtina
Zdroj: World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2022 May 31; Vol. 38 (7), pp. 123. Date of Electronic Publication: 2022 May 31.
DOI: 10.1007/s11274-022-03304-6
Abstrakt: Epsilon-poly-L-lysine (ε-PL) is an unusual biopolymer composed of L-lysine produced by several microorganisms, especially by the genus Streptomyces. Due to its excellent antimicrobial activity, good water solubility, high safety, and biodegradable nature, ε-PL with a GRAS status has been widely used in food and pharmaceutical industries. In the past years, studies have focused on the biotechnological production of ɛ-PL, the biosynthetic mechanism of microbial ɛ-PL, and its application. To provide new perspectives from recent advances, the review introduced the methods for the isolation of ɛ-PL producing strains and the biosynthetic mechanism of microbial ɛ-PL. We summarized the strategies for the improvement of ɛ-PL producing strains, including physical and chemical mutagenesis, ribosome engineering and gene engineering, and compared the different metabolic regulation strategies for improving ɛ-PL production, including medium optimization, nutrient supply, pH control, and dissolved oxygen control. Then, the downstream purification methods of ɛ-PL and its recent applications in food and medicine industries were introduced. Finally, we also proposed the potential challenges and the perspectives for the production of ε-PL.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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