Membrane elasticity modulated by cholesterol in model of porcine eye lens-lipid membrane.
Autor: | Khadka NK; Department of Physics, Boise State University, Boise, ID, USA., Mortimer MF; Department of Physics, Boise State University, Boise, ID, USA., Marosvari M; Department of Physics, Boise State University, Boise, ID, USA., Timsina R; Department of Physics, Boise State University, Boise, ID, USA., Mainali L; Department of Physics, Boise State University, Boise, ID, USA; Biomolecular Sciences Graduate Program, Boise State University, Boise, ID, USA. Electronic address: laxmanmainali@boisestate.edu. |
---|---|
Jazyk: | angličtina |
Zdroj: | Experimental eye research [Exp Eye Res] 2022 Jul; Vol. 220, pp. 109131. Date of Electronic Publication: 2022 May 27. |
DOI: | 10.1016/j.exer.2022.109131 |
Abstrakt: | Experimental evidence shows that the eye lens loses its elasticity dramatically with age. It has also been reported that the cholesterol (Chol) content in the eye lens fiber cell plasma membrane increases significantly with age. High Chol content leads to the formation of cholesterol bilayer domains (CBDs) in the lens membrane. The role of high Chol associated with lens elasticity is unclear. The purpose of this research is to investigate the membrane elasticity of the model of porcine lens-lipid (MPLL) membrane with increasing Chol content to elucidate the role of high Chol in lens membrane elasticity. In this study, we used atomic force microscopy (AFM) to study the mechanical properties (breakthrough force and area compressibility modulus (K (Copyright © 2022 Elsevier Ltd. All rights reserved.) |
Databáze: | MEDLINE |
Externí odkaz: |