Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?

Autor: Merkuleva IA; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Shcherbakov DN; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Borgoyakova MB; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Isaeva AA; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Nesmeyanova VS; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Volkova NV; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Aripov VS; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Shanshin DV; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Karpenko LI; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Belenkaya SV; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Kazachinskaia EI; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Volosnikova EA; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Esina TI; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Sergeev AA; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Titova KA; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Konyakhina YV; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Zaykovskaya AV; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Pyankov OV; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Kolosova EA; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia.; Russian-American Anti-Cancer Center, Altai State University, 656049 Barnaul, Russia., Viktorina OE; Russian-American Anti-Cancer Center, Altai State University, 656049 Barnaul, Russia., Shelemba AA; Federal Research Center of Fundamental and Translational Medicine, 630060 Novosibirsk, Russia., Rudometov AP; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia., Ilyichev AA; State Research Center of Virology and Biotechnology 'Vector', Rospotrebnadzor, World-Class Genomic Research Center for Biological Safety and Technological Independence, Federal Scientific and Technical Program on the Development of Genetic Technologies, 630559 Koltsovo, Russia.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2022 May 16; Vol. 14 (5). Date of Electronic Publication: 2022 May 16.
DOI: 10.3390/v14051060
Abstrakt: Currently, SARS-CoV-2 spike receptor-binding-domain (RBD)-based vaccines are considered one of the most effective weapons against COVID-19. During the first step of assessing vaccine immunogenicity, a mouse model is often used. In this paper, we tested the use of five experimental animals (mice, hamsters, rabbits, ferrets, and chickens) for RBD immunogenicity assessments. The humoral immune response was evaluated by ELISA and virus-neutralization assays. The data obtained show hamsters to be the least suitable candidates for RBD immunogenicity testing and, hence, assessing the protective efficacy of RBD-based vaccines.
Databáze: MEDLINE
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