4-Dimethylaminoantipyrine as a Broad Electrochemical Indicator for Immunosensors Platform.

Autor: Melo FCC; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Avenida Amazonas s/n Bl. 2E, sl. 248, Uberlândia 38402-022, Minas Gerais, Brazil., Alves RP; Biological Science, Universidade Federal do Triângulo Mineiro, Avenida Antônio Baiano, 150, Cidade Nova, Iturama 38280-000, Minas Gerais, Brazil., Valle AL; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Avenida Amazonas s/n Bl. 2E, sl. 248, Uberlândia 38402-022, Minas Gerais, Brazil., Santos FAA; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Avenida Amazonas s/n Bl. 2E, sl. 248, Uberlândia 38402-022, Minas Gerais, Brazil., Dias ACS; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Avenida Amazonas s/n Bl. 2E, sl. 248, Uberlândia 38402-022, Minas Gerais, Brazil., Goulart IMB; National Reference Center for Sanitary Dermatology and Leprosy, Clinics Hospital, School of Medicine, Federal University of Uberlândia, R. Capricórnio, 94-Jardim Brasília, Uberlândia 38401-404, Minas Gerais, Brazil., Oliveira EGA; Institute of Chemistry, Federal University of Uberlândia, Avenida João Naves de Ávila, 2121, Bloco 5T, sala 204, Uberlândia 38400-902, Minas Gerais, Brazil., Oliveira GS; Institute of Chemistry, Federal University of Uberlândia, Avenida João Naves de Ávila, 2121, Bloco 5T, sala 204, Uberlândia 38400-902, Minas Gerais, Brazil., Rodrigues LP; Institute of Engineering Science and Technology, Federal University of the Jequitinhonha and Mucuri Valleys, Av. Um, n. 4.050-Cidade Universitária, Janaúba 39447-790, Minas Gerais, Brazil., Goulart LR; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlândia, Avenida Amazonas s/n Bl. 2E, sl. 248, Uberlândia 38402-022, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Sensors (Basel, Switzerland) [Sensors (Basel)] 2022 May 12; Vol. 22 (10). Date of Electronic Publication: 2022 May 12.
DOI: 10.3390/s22103681
Abstrakt: Here, we describe 4-dimethylaminoantipyrine (4-DMAA)-mediated interfacing as a broad biochemical indicator to stabilize and promote the higher response of electrodes for immunological detection. We hypothesized that the improved biological interactions of 4-DMAA with electrodes and biological samples may be due to the interaction properties of the benzene and pyrazole chemical groups with graphite and proteins, respectively. In order to demonstrate that 4-DMAA could be used as a general indicator in electrochemical immunoassays, we used peptides as probes for the diagnosis of four neglected tropical infectious diseases Tegumentary leishmaniasis , Visceral leishmaniasis , Strongyloidiasis , and Leprosy on commercial graphite screen-printed electrodes. 4-DMAA oxidation was used to indicate specific biological recognition between the epitope-based peptide and serum immunoglobulin G (IgG) from infected patients. We demonstrated that 4-DMAA should be incorporated into the electrodes prior to serum application, which avoids interference with its sensitivity and specificity. In addition, 4-DMAA oxidizes at a low anodic potential, and the oxidation peak is useful for detecting proteins in biological fluids. In summary, we have successfully demonstrated the broad application of 4-DMAA as a general indicator for the specific diagnosis of four infectious diseases in electrochemical immunosensors. Such a strategy is quite advantageous for indirect detection of proteins that lack electrochemical activities or are spatially inaccessible on the electrode surface. This new indicator opens a new avenue for monitoring biological recognition, especially for immunosensors.
Databáze: MEDLINE
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