Reactivity of a Recombinant Esterase from Thermus thermophilus HB27 in Aqueous and Organic Media.

Autor:	González-González R; Biochemistry Laboratory, CITACA-Agri-Food Research and Transfer Cluster, Campus Auga, Universidade de Vigo, 32004 Ourense, Spain., Fuciños P; Biochemistry Laboratory, CITACA-Agri-Food Research and Transfer Cluster, Campus Auga, Universidade de Vigo, 32004 Ourense, Spain., Beneventi E; GalChimia, SA, Parque Empresarial de Touro, Parcelas 26-27, Fonte Díaz, 15822 Touro, Spain., López-López O; Grupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Facultade de Ciencias, Universidade da Coruña, 15071 A Coruña, Spain., Pampín B; GalChimia, SA, Parque Empresarial de Touro, Parcelas 26-27, Fonte Díaz, 15822 Touro, Spain., Rodríguez R; GalChimia, SA, Parque Empresarial de Touro, Parcelas 26-27, Fonte Díaz, 15822 Touro, Spain., González-Siso MI; Grupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Facultade de Ciencias, Universidade da Coruña, 15071 A Coruña, Spain., Cruces J; GalChimia, SA, Parque Empresarial de Touro, Parcelas 26-27, Fonte Díaz, 15822 Touro, Spain., Rúa ML; Biochemistry Laboratory, CITACA-Agri-Food Research and Transfer Cluster, Campus Auga, Universidade de Vigo, 32004 Ourense, Spain.
Jazyk:	angličtina
Zdroj:	Microorganisms [Microorganisms] 2022 Apr 27; Vol. 10 (5). Date of Electronic Publication: 2022 Apr 27.
DOI:	10.3390/microorganisms10050915
Abstrakt:	The thermoalkalophilic membrane-associated esterase E34Tt from Thermus thermophilus HB27 was cloned and expressed in Kluyveromyces lactis (KLEST-3S esterase). The recombinant enzyme was tested as a biocatalyst in aqueous and organic media. It displayed a high thermal stability and was active in the presence of 10% ( v / v ) organic solvents and 1% ( w / v ) detergents. KLEST-3S hydrolysed triglycerides of various acyl chains, which is a rare characteristic among carboxylic ester hydrolases from extreme thermophiles, with maximum activity on tributyrin. It also displayed interfacial activation towards triacetin. KLEST-3S was also tested as a biocatalyst in organic media. The esterase provided high yields for the acetylation of alcohols. In addition, KLEST-3S catalyzed the stereoselective hydrolysis of ( R , S )-ibuprofen methyl ester (87% ee). Our results indicate that KLEST-3S may be a robust and efficient biocatalyst for application in industrial bioconversions.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu