Patients' Stem Cells Differentiation in a 3D Environment as a Promising Experimental Tool for the Study of Amyotrophic Lateral Sclerosis.

Autor: Scarian E; Department of Brain and Behavioral Sciences, University of Pavia, Via Forlanini 6, 27100 Pavia, Italy.; Cellular Models and Neuroepigenetics Unit, IRCCS Mondino Foundation, via Mondino 2, 27100 Pavia, Italy., Bordoni M; Cellular Models and Neuroepigenetics Unit, IRCCS Mondino Foundation, via Mondino 2, 27100 Pavia, Italy., Fantini V; Department of Brain and Behavioral Sciences, University of Pavia, Via Forlanini 6, 27100 Pavia, Italy.; Laboratory of Neurobiology and Neurogenetic, Golgi-Cenci Foundation, Corso San Martino 10, 20081 Abbiategrasso, Italy., Jacchetti E; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta', Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy., Raimondi MT; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta', Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy., Diamanti L; Neuroncology Unit, IRCCS Mondino Foundation, via Mondino 2, 27100 Pavia, Italy., Carelli S; Pediatric Clinical Research Center 'Fondazione Romeo ed Enrica Invernizzi', Department of Biomedical and Clinical Sciences 'L. Sacco', University of Milan, Via Giovanni Battista Grassi, 74, 20157 Milan, Italy., Cereda C; Genomic and Post-Genomic Unit, IRCCS Mondino Foundation, via Mondino 2, 27100 Pavia, Italy.; Neonatal Screening and Metabolic Disorders Unit, Department of Woman, Mother and Neonate, 'V. Buzzi' Children's Hospital-ASST Fatebenefratelli Sacco, Via Lodovico Castelvetro, 52, 20154 Milan, Italy., Pansarasa O; Cellular Models and Neuroepigenetics Unit, IRCCS Mondino Foundation, via Mondino 2, 27100 Pavia, Italy.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 May 11; Vol. 23 (10). Date of Electronic Publication: 2022 May 11.
DOI: 10.3390/ijms23105344
Abstrakt: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease (NDD) that affects motor neurons, causing weakness, muscle atrophy and spasticity. Unfortunately, there are only symptomatic treatments available. Two important innovations in recent years are three-dimensional (3D) bioprinting and induced pluripotent stem cells (iPSCs). The aim of this work was to demonstrate the robustness of 3D cultures for the differentiation of stem cells for the study of ALS. We reprogrammed healthy and sALS peripheral blood mononuclear cells (PBMCs) in iPSCs and differentiated them in neural stem cells (NSCs) in 2D. NSCs were printed in 3D hydrogel-based constructs and subsequently differentiated first in motor neuron progenitors and finally in motor neurons. Every step of differentiation was tested for cell viability and characterized by confocal microscopy and RT-qPCR. Finally, we tested the electrophysiological characteristics of included NSC34. We found that NSCs maintained good viability during the 3D differentiation. Our results suggest that the hydrogel does not interfere with the correct differentiation process or with the electrophysiological features of the included cells. Such evidence confirmed that 3D bioprinting can be considered a good model for the study of ALS pathogenesis.
Databáze: MEDLINE
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