First Report of an Asymptomatic Leishmania (Viannia) shawi Infection Using a Nasal Swab in Amazon, Brazil.

Autor:	Oliveira LP; Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil., Nascimento LCS; Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil., Santos FS; Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil., Takamatsu JLC; Hospital Municipal de Tomé Açu, Tomé-Açu 68680-000, PA, Brazil., Sanchez LRP; Hospital Municipal de Tomé Açu, Tomé-Açu 68680-000, PA, Brazil., Santos WS; Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil., Garcez LM; Ministério da Saúde, Secretaria de Vigilância em Saúde, Instituto Evandro Chagas, Ananindeua 67015-120, PA, Brazil.; Departamento de Patologia, Universidade do Estado do Pará, Belém 66050-540, PA, Brazil.
Jazyk:	angličtina
Zdroj:	International journal of environmental research and public health [Int J Environ Res Public Health] 2022 May 23; Vol. 19 (10). Date of Electronic Publication: 2022 May 23.
DOI:	10.3390/ijerph19106346
Abstrakt:	The state of Pará has recorded seven Leishmania species that cause tegumentary leishmaniasis (TL). Leishmania species induce distinct immunological responses from the host and exhibit resistance to Glucantime, the first-line drug treatment for TL in Brazil. Objective: Identify the etiology of TL in an Amazonian city in the state of Pará. Material and Methods: Eleven patients with TL were recruited and nasal swabs, lesion swabs, and skin fragments samples were collected. In the control group (n = 6), only the nasal swabs were collected. Polymerase Chain Reaction (PCR) amplification of the gene region hsp70-234 was performed using the extracted DNA from the samples, from which nine patients with TL and five in the control group were positive. Products were sequenced, mounted in CAP3 software, aligned using MAFFT v.7.221, edited in Geneious software v.8.1.7, and compared and aligned with sequences available in GenBank using the BLAST tool. Results: For patients with TL, six molecular diagnosis at the species level ( L. ( Viannia ) braziliensis (n = 5/9), L. ( Viannia ) shawi (n = 1/9)) and three at the genus level ( Leishmania sp. (n = 3/9)) were obtained. In the control group, four individuals were infected with Leishmania sp. (n = 4/5) and L. (V.) shawi (n = 1/5). Conclusion: This is the first report of L. (V.) shawi infection in the mucosal secretion of a healthy person in Brazil. Moreover, genetic variants were identified in the haplotypes of L. (V.) braziliensis in the gene sequence hsp70-234.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu