Clinical Evaluation of Nasopharyngeal, Oropharyngeal, Nasal Swabs, and Saliva for the Detection of SARS-CoV-2 by Direct RT-PCR.

Autor: Kiryanov SA; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named after Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia., Levina TA; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named after Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia.; LLC 'DNA-Technology', 117587 Moscow, Russia., Kadochnikova VV; LLC 'DNA-Technology', 117587 Moscow, Russia., Konopleva MV; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named after Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia., Suslov AP; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named after Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia., Trofimov DY; LLC 'DNA-Technology', 117587 Moscow, Russia.
Jazyk: angličtina
Zdroj: Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2022 Apr 27; Vol. 12 (5). Date of Electronic Publication: 2022 Apr 27.
DOI: 10.3390/diagnostics12051091
Abstrakt: Nasopharyngeal swab (NPS) and oropharyngeal swab (OPS) are the most widely used upper respiratory tract specimens for diagnosis of SARS-CoV-2 using RT-qPCR. In contrast, nasal swab (NS) and saliva (SS), recently recommended by the WHO, are rarely used, and their test accuracy is limited. The method for direct RT-PCR detection of SARS-CoV-2 does not require an RNA extraction and is faster and easier than standard RT-PCR tests with RNA extraction. This study aimed to compare the diagnostic performance of upper respiratory tract samples for SARS-CoV-2 detection using the direct RT-PCR without preliminary heat inactivation. Here we report the application and validation of direct RT-PCR SARS-CoV-2 RNA on 165 clinical specimens of NPS/OP, and 36 samples of NS/NPS and 37 saliva samples (for the latter with prior deproteinization). The overall sensitivity estimates were 95.9%, 94.2%, 88.9%, and 94.6% for NPS/OPS/NS/SS samples, respectively, and the specificity was 100% against standard RT-PCR with RNA extraction. Overall, NS and SS testing by direct RT-PCR had sufficient sensitivity to detect SARS-CoV-2. They can be acceptable alternative to NPS/OPS for rapid detection of SARS-CoV-2 infections in future.
Databáze: MEDLINE
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