| Autor: |
Pankok F; Institute for Infection Control and Infectious Diseases, University Medical Center Göttingen, 37075 Göttingen, Germany., Taudien S; Institute for Infection Control and Infectious Diseases, University Medical Center Göttingen, 37075 Göttingen, Germany., Dekker D; Department of Implementation Research, Bernhard Nocht Institute for Tropical Medicine Hamburg, 20359 Hamburg, Germany., Thye T; Department of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine Hamburg, 20359 Hamburg, Germany., Oppong K; Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi 039-5028, Ghana., Wiafe Akenten C; Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi 039-5028, Ghana., Lamshöft M; Department of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine Hamburg, 20359 Hamburg, Germany.; German Center for Infection Research (DZIF), Partner Site Hamburg-Lübeck-Borstel-Riems, 80331 Munich, Germany., Jaeger A; Department of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine Hamburg, 20359 Hamburg, Germany., Kaase M; Institute for Infection Control and Infectious Diseases, University Medical Center Göttingen, 37075 Göttingen, Germany., Scheithauer S; Institute for Infection Control and Infectious Diseases, University Medical Center Göttingen, 37075 Göttingen, Germany., Tanida K; Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, External Site at the Bernhard Nocht Institute for Tropical Medicine Hamburg, 20359 Hamburg, Germany., Frickmann H; Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, External Site at the Bernhard Nocht Institute for Tropical Medicine Hamburg, 20359 Hamburg, Germany.; Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany., May J; Department of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine Hamburg, 20359 Hamburg, Germany.; German Center for Infection Research (DZIF), Partner Site Hamburg-Lübeck-Borstel-Riems, 80331 Munich, Germany.; Tropical Medicine II, University Medical Center Hamburg-Eppendorf (UKE), 20251 Hamburg, Germany., Loderstädt U; Institute for Infection Control and Infectious Diseases, University Medical Center Göttingen, 37075 Göttingen, Germany. |
| Abstrakt: |
Little information is available on the local epidemiology of mobile genetic elements such as plasmids harboring acquired beta-lactamase genes in Western African Ghana. In the present study, we screened for plasmids in three Escherichia coli and four Klebsiella pneumoniae isolates expressing extended spectrum beta-lactamases (ESBL) mediated by the bla CTX-M-15 gene from chronically infected wounds of Ghanaian patients. Bacterial isolates were subjected to combined short-read and long-read sequencing to obtain the sequences of their respective plasmids. In the bla CTX-M-15 -gene-carrying plasmids of the four ESBL-positive K. pneumoniae isolates, IncFIB/IncFII ( n = 3) and FIA ( n = 1) sequences were detected, while in the bla CTX-M-15 -gene-carrying plasmids of the three ESBL-positive E. coli isolates, IncFIA/IncFIB ( n = 2) and IncFIB ( n = 1) sequences were found. The three IncFIB/IncFII sequence-containing plasmids were almost identical to a K. pneumoniae plasmid reported from France. They belonged to the clonal lineages ST17, ST36 and ST39 of K. pneumoniae , suggesting transversal spread of this obviously evolutionary successful plasmid in Ghana. Other resistance gene-encoding plasmids observed in the assessed Enterobacterales harbored IncFIA/IncR and IncFII sequences. International spread was confirmed by the high genetic similarity to resistance-mediating plasmids published from Asia, Australia, Europe and Northern America, including a bla CTX-M-15 -gene-carrying plasmid isolated from a wild bird in Germany. In conclusion, the study contributed to the scarcely available information on the epidemiology of third-generation cephalosporine resistance-mediating plasmids in Ghana. Furthermore, the global spread of resistance-mediating plasmids provided hints on the evolutionary success of individual resistance-harboring plasmids by transversal spread among K. pneumoniae lineages in Ghana. |
