Lasiodiplodia theobromae as a causal pathogen of leaf blight, stem canker, and pod rot of Theobroma cacao in Malaysia.

Autor: Huda-Shakirah AR; School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia., Mohamed Nor NMI; School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia., Zakaria L; School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia., Leong YH; National Poison Centre, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia., Mohd MH; School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia. masratulhawa@usm.my.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 May 27; Vol. 12 (1), pp. 8966. Date of Electronic Publication: 2022 May 27.
DOI: 10.1038/s41598-022-13057-9
Abstrakt: Symptoms of leaf blight, stem canker, and pod rot were observed on T. cacao during a series of samplings conducted in several states of Malaysia from September 2018 to March 2019. The identity of the pathogen that was responsible for the diseases was determined using morphological characteristics, DNA sequences, and phylogenetic analyses of multiple genes, namely, internal transcribed spacer (ITS), elongation translation factor 1-alpha (tef1-α), β-tubulin (tub2), and RNA polymerase subunit II (rpb2). A total of 57 isolates recovered from diseased leaves of T. cacao (13 isolates), stems (20 isolates), and pods (24 isolates) showed morphological features that resembled Lasiodiplodia sp. The identity of the isolates was further determined up to the species level by comparing DNA sequences and phylogenetic analyses of multiple genes. The phylogenetic analysis of the combined dataset of ITS, tef1-α, tub2, and rpb2 elucidated that all of the isolates obtained were Lasiodiplodia theobromae as supported by 97% bootstrap value. The results of pathogenicity tests revealed L. theobromae as the causal pathogen of leaf blight, stem canker, and pod rot of T. cacao.
(© 2022. The Author(s).)
Databáze: MEDLINE
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