Quality of MALDI-TOF mass spectra in routine diagnostics: results from an international external quality assessment including 36 laboratories from 12 countries using 47 challenging bacterial strains.

Autor:	Cuénod A; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland; Division of Clinical Bacteriology and Mycology, University Hospital Basel, Basel, Switzerland. Electronic address: aline.cuenod@stud.unibas.ch., Aerni M; Labor Team W, Goldach, Switzerland., Bagutti C; State Laboratory Basel-Stadt, Basel, Switzerland., Bayraktar B; University of Health Sciences, Sisli Hamidiye Etfal Teaching and Research Hospital, Istanbul, Turkey., Boz ES; Department of Medical Microbiology, University of Health Sciences, Haydarpasa Numune Teaching and Research Hospital, Istanbul, Turkey., Carneiro CB; University Hospital Freiburg, Freiburg im Breisgau, Germany., Casanova C; Institute for Infectious Diseases, University of Bern, Bern, Switzerland., Coste AT; Institute of Microbiology, University Hospital Lausanne, Lausanne, Switzerland., Damborg P; Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark., van Dam DW; Zuyderland MC, Sittard, the Netherlands., Demirci M; Department of Medical Microbiology, Kirklareli University, Kirklareli, Turkey., Drevinek P; Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czech Republic., Dubuis O; Viollier AG, Allschwil, Switzerland., Fernandez J; Division of Laboratory Medicine, Laboratory of Bacteriology, University Hospital of Geneva, Geneva, Switzerland., Greub G; Institute of Microbiology, University Hospital Lausanne, Lausanne, Switzerland., Hrabak J; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Plzen, Czech Republic., Hürkal Yiğitler G; University of Health Sciences, Sisli Hamidiye Etfal Teaching and Research Hospital, Istanbul, Turkey., Hurych J; Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czech Republic., Jensen TG; Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark., Jost G; Dianalabs, Geneva, Switzerland., Kampinga GA; Department of Medical Microbiology and Infection Prevention, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands., Kittl S; Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland., Lammens C; Department of Medical Microbiology, University of Antwerp, Belgium., Lang C; Viollier AG, Allschwil, Switzerland., Lienhard R; ADMED Microbiologie, La Chaux de Fonds, Switzerland., Logan J; Reference Services Division, UK Health Security Agency, London, United Kingdom., Maffioli C; MCL Laboratories, Niederwangen, Switzerland., Mareković I; Department of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, Zagreb, Croatia., Marschal M; Institute of Medical Microbiology and Hygiene, University of Tübingen, Tübingen, Germany., Moran-Gilad J; School of Public Health, Ben Gurion University of the Negev and Soroka University Medical Center, Beer Sheva, Israel., Nolte O; Center for Laboratory Medicine, St. Gallen, Switzerland., Oberle M; Cantonal Hospital Aarau, Aarau, Switzerland., Pedersen M; Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark., Pflüger V; Mabritec AG, Riehen, Switzerland., Pranghofer S; Bioanalytica AG, Lucerne, Switzerland., Reichl J; Austrian Agency for Health and Food Safety, Vienna, Austria., Rentenaar RJ; UMC Utrecht, Utrecht, the Netherlands., Riat A; Division of Laboratory Medicine, Laboratory of Bacteriology, University Hospital of Geneva, Geneva, Switzerland., Rodríguez-Sánchez B; Hospital General Universitario Gregorio Maranon, Madrid, Spain., Schilt C; ADMED Microbiologie, La Chaux de Fonds, Switzerland., Schlotterbeck AK; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland., Schrenzel J; Division of Laboratory Medicine, Laboratory of Bacteriology, University Hospital of Geneva, Geneva, Switzerland., Troib S; School of Public Health, Ben Gurion University of the Negev and Soroka University Medical Center, Beer Sheva, Israel., Willems E; Clinical Laboratory AZNikolaas, Sint-Niklaas, Belgium., Wootton M; University Hospital of Wales, Cardiff, United Kingdom., Ziegler D; Eurofins Scientific AG, Schönenwerd, Switzerland., Egli A; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland; Division of Clinical Bacteriology and Mycology, University Hospital Basel, Basel, Switzerland.
Jazyk:	angličtina
Zdroj:	Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2023 Feb; Vol. 29 (2), pp. 190-199. Date of Electronic Publication: 2022 May 25.
DOI:	10.1016/j.cmi.2022.05.017
Abstrakt:	Objectives: Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is a widely used method for bacterial species identification. Incomplete databases and mass spectral quality (MSQ) still represent major challenges. Important proxies for MSQ are the number of detected marker masses, reproducibility, and measurement precision. We aimed to assess MSQs across diagnostic laboratories and the potential of simple workflow adaptations to improve it. Methods: For baseline MSQ assessment, 47 diverse bacterial strains, which are challenging to identify by MALDI-TOF MS, were routinely measured in 36 laboratories from 12 countries, and well-defined MSQ features were used. After an intervention consisting of detailed reported feedback and instructions on how to acquire MALDI-TOF mass spectra, measurements were repeated and MSQs were compared. Results: At baseline, we observed heterogeneous MSQ between the devices, considering the median number of marker masses detected (range = [2-25]), reproducibility between technical replicates (range = [55%-86%]), and measurement error (range = [147 parts per million (ppm)-588 ppm]). As a general trend, the spectral quality was improved after the intervention for devices, which yielded low MSQs in the baseline assessment as follows: for four out of five devices with a high measurement error, the measurement precision was improved (p-values <0.001, paired Wilcoxon test); for six out of ten devices, which detected a low number of marker masses, the number of detected marker masses increased (p-values <0.001, paired Wilcoxon test). Discussion: We have identified simple workflow adaptations, which, to some extent, improve MSQ of poorly performing devices and should be considered by laboratories yielding a low MSQ. Improving MALDI-TOF MSQ in routine diagnostics is essential for increasing the resolution of bacterial identification by MALDI-TOF MS, which is dependent on the reproducible detection of marker masses. The heterogeneity identified in this external quality assessment (EQA) requires further study. (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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