Ionizing radiation induced DNA damage via ROS production in nano ozonized oil treated B-16 melanoma and OV-90 ovarian cells.

Autor: Yalçın Y; Department of Nanotechnology Engineering, Zonguldak Bülent Ecevit University, Incivez, Zonguldak, 67100, Turkey., Tekin İÖ; Department of Immunology, Zonguldak Bülent Ecevit University, School of Medicine, Zonguldak, 67100, Turkey., Tığlı Aydın RS; Department of Nanotechnology Engineering, Zonguldak Bülent Ecevit University, Incivez, Zonguldak, 67100, Turkey; Department of Biomedical Engineering, Zonguldak Bülent Ecevit University, Incivez, Zonguldak, 67100, Turkey. Electronic address: rseda.tigli@gmail.com.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2022 Jul 30; Vol. 615, pp. 143-149. Date of Electronic Publication: 2022 May 13.
DOI: 10.1016/j.bbrc.2022.05.030
Abstrakt: In this study, we aimed to investigate ozonized oil nanoemulsions (OZNEs) as a radiosensitizer within B-16 melanoma and OV-90 ovarian cells under X-ray irradiation in vitro. Radiation sensitivity of OZNE treated B-16 melanoma cells and OV-90 ovarian cells were evaluated by performing cell cycle analysis, Reactive Oxygen Species (ROS) and ɣ-H2AX assays by flow cytometry. OZNEs induced G0-1 phase arrest of B-16 melanoma cells for all radiation doses and G2/M arrest for 8 Gy and 15 Gy doses. OZNE treated B-16 melanoma and OV-90 ovarian cells induced DNA damage via the increase in ROS production, as well as significant increase in the expression of ɣ-H2AX under even low doses of radiation (2 Gy). Thus, OZNEs are suggested to help to optimize cancer RT as a radiosensitizer and further studies will significantly outperform recent advances in this field.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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