| Autor: |
Shinya U; Kagoshima Agriculture Mutual Aid Association Soo Branch, Soo 899-8212, Japan., Iwamura Y; Kagoshima Agriculture Mutual Aid Association Soo Branch, Soo 899-8212, Japan., Yamato O; Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan., Pambudi D; Department of Mathematics Education, Faculty of Teacher Training and Education, Sebelas Maret University, Surakata 57126, Indonesia., Widodo OS; Joint Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.; Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia., Taniguchi M; Laboratory of Theriogenology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan., Takagi M; Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia.; Laboratory of Theriogenology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753-8515, Japan. |
| Abstrakt: |
The present study aimed to compare serum amyloid A (SAA) concentrations of Japanese Black (JB) breeding cows in both clinically normal and diseased cows diagnosed by veterinarians using modified latex agglutination turbidimetric immunoassay (LATIA) to determine the cut-off values for healthy and diseased JB cows. For the comparison, a total of 289 serum samples of healthy cows without any clinical symptoms intended for the metabolic profile test and 66 serums from diseased cows clinically diagnosed by veterinarians were measured for the SAA concentrations. A significant difference ( p -value = 6.68 × 10 -29 ) was observed in the mean SAA concentrations between the healthy (2.8 ± 3.2 mg/L) and diseased (54.8 ± 76.8 mg/L) groups, and the median concentrations of the healthy and diseased groups were 1.5 mg/L and 31.2 mg/L, respectively. Finally, the cut-off SAA concentrations at each probability were 2.9 mg/L ( p = 0.05), 5.7 mg/L ( p = 0.1), 13.7 mg/L ( p = 0.5), and 21.8 mg/L ( p = 0.9), respectively, and 6.5 mg/L ( p = 0.122) based on evaluation performed using the receiver operating characteristic curve. The results indicated that, with the practical application of the obtained cut-off value, the measurement of SAA concentrations for JB breeding cows with LATIA could be potentially beneficial in the early evaluation of inflammatory diseases in JB breeding cows and possibly useful in the prevention of not only metabolic diseases but also non-nutritional diseases during the perinatal period of JB breeding cows. |
