Combined Analysis of Transcriptome and T-Cell Receptor Alpha and Beta (TRA /TRB ) Repertoire in Paucicellular Samples at the Single-Cell Level.

Autor:	Litjens NHR; Erasmus MC Transplant Institute, Division of Nephrology and Transplantation, Department of Internal Medicine, Erasmus MC University Medical Center, Rotterdam, The Netherlands., Langerak AW; Department of Immunology, Laboratory Medical Immunology, Erasmus MC University Medical Center, Rotterdam, The Netherlands., Azmani Z; Center for Biomics, Erasmus MC University Medical Center, Rotterdam, The Netherlands.; Department of Cell Biology, Erasmus MC University Medical Center, Rotterdam, The Netherlands., den Dekker X; Center for Biomics, Erasmus MC University Medical Center, Rotterdam, The Netherlands.; Department of Cell Biology, Erasmus MC University Medical Center, Rotterdam, The Netherlands., Betjes MGH; Erasmus MC Transplant Institute, Division of Nephrology and Transplantation, Department of Internal Medicine, Erasmus MC University Medical Center, Rotterdam, The Netherlands., Brouwer RWW; Center for Biomics, Erasmus MC University Medical Center, Rotterdam, The Netherlands.; Department of Cell Biology, Erasmus MC University Medical Center, Rotterdam, The Netherlands., van IJcken WFJ; Center for Biomics, Erasmus MC University Medical Center, Rotterdam, The Netherlands. w.vanijcken@erasmusmc.nl.; Department of Cell Biology, Erasmus MC University Medical Center, Rotterdam, The Netherlands. w.vanijcken@erasmusmc.nl.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2453, pp. 231-259.
DOI:	10.1007/978-1-0716-2115-8_14
Abstrakt:	With the advent of next-generation sequencing (NGS) methodologies, the total repertoires of B and T cells can be disclosed in much more detail than ever before. Even though many of these strategies do provide in-depth and high-resolution information of the immunoglobulin (IG) and/or T-cell receptor (TR) repertoire, one clear disadvantage is that the IG/TR profiles cannot be connected to individual cells. Single-cell technologies do allow to study the IG/TR repertoire at the individual cell level. This is especially relevant in cell samples in which much heterogeneity of the cell population is expected. By combining the IG/TR repertoire with transcriptome data, the reactivity of the B or T cell can be associated with activation or maturation stages. An additional advantage of such single-cell technologies is that the combination of both IG and both TR chains can be studied on a per cell basis, which better reflects the antigen receptor reactivity of cells. Here we present the ICELL8 single-cell method for the parallel analysis of the TR repertoire and transcriptome, which is especially useful in samples that contain relatively few cells. (© 2022. The Author(s).)
Databáze:	MEDLINE
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