Native RNA G quadruplex immunoprecipitation (rG4IP) from mammalian cells.
| Autor: | Surani AA; John van Geest Cancer Research Centre, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK., Montiel-Duarte C; John van Geest Cancer Research Centre, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2022 May 20; Vol. 3 (2), pp. 101372. Date of Electronic Publication: 2022 May 20 (Print Publication: 2022). |
| DOI: | 10.1016/j.xpro.2022.101372 |
| Abstrakt: | G quadruplex structures play an important role in regulating DNA replication and transcription and mRNA translation. Although there are several techniques that can determine its formation in vitro , the study of RNA G quadruplexes in vivo is not simple. In the current protocol, we describe an optimized technique (RNA G quadruplex immunoprecipitation [rG4IP]) to selectively pull down native cytoplasmic RNAs containing G quadruplex structures in mammalian cells. We also use a bicistronic plasmid to confirm and pinpoint the structure location. For complete details on the use and execution of this protocol, please refer to Surani et al. (2022). Competing Interests: The authors declare no competing interests. (© 2022 The Author(s).) |
| Databáze: | MEDLINE |
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