Electrically controlling and optically observing the membrane potential of supported lipid bilayers.

Autor:	Yudovich S; Department of Physics, Bar-Ilan University, Ramat-Gan, Israel; Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel. Electronic address: shimon.yudovich@gmail.com., Marzouqe A; Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel; Department of Chemistry, Bar-Ilan University, Ramat-Gan, Israel., Kantorovitsch J; Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel., Teblum E; Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel., Chen T; Third Institute of Physics-Biophysics, Georg August University, Göttingen, Germany., Enderlein J; Third Institute of Physics-Biophysics, Georg August University, Göttingen, Germany; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), Georg August University, Göttingen, Germany., Miller EW; Departments of Chemistry, Molecular & Cell Biology, and Helen Wills Neuroscience Institute, University of California, Berkeley, California., Weiss S; Department of Physics, Bar-Ilan University, Ramat-Gan, Israel; Institute for Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel; Departments of Chemistry and Biochemistry, Physiology, and California NanoSystems Institute, University of California Los Angeles, Los Angeles, California. Electronic address: sweiss@chem.ucla.edu.
Jazyk:	angličtina
Zdroj:	Biophysical journal [Biophys J] 2022 Jul 05; Vol. 121 (13), pp. 2624-2637. Date of Electronic Publication: 2022 May 25.
DOI:	10.1016/j.bpj.2022.05.037
Abstrakt:	Supported lipid bilayers are a well-developed model system for the study of membranes and their associated proteins, such as membrane channels, enzymes, and receptors. These versatile model membranes can be made from various components, ranging from simple synthetic phospholipids to complex mixtures of constituents, mimicking the cell membrane with its relevant physiochemical and molecular phenomena. In addition, the high stability of supported lipid bilayers allows for their study via a wide array of experimental probes. In this work, we describe a platform for supported lipid bilayers that is accessible both electrically and optically, and demonstrate direct optical observation of the transmembrane potential of supported lipid bilayers. We show that the polarization of the supported membrane can be electrically controlled and optically probed using voltage-sensitive dyes. Membrane polarization dynamics is understood through electrochemical impedance spectroscopy and the analysis of an equivalent electrical circuit model. In addition, we describe the effect of the conducting electrode layer on the fluorescence of the optical probe through metal-induced energy transfer, and show that while this energy transfer has an adverse effect on the voltage sensitivity of the fluorescent probe, its strong distance dependency allows for axial localization of fluorescent emitters with ultrahigh accuracy. We conclude with a discussion on possible applications of this platform for the study of voltage-dependent membrane proteins and other processes in membrane biology and surface science. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2022 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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