In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella.

Autor:	Koczerka M; INRAE, Université de Tours, ISP, Nouzilly, France., Lantier I; INRAE, Université de Tours, ISP, Nouzilly, France., Pinard A; INRAE, PFIE , Nouzilly, France., Morillon M; INRAE, Université de Tours, ISP, Nouzilly, France., Deperne J; INRAE, Université de Tours, ISP, Nouzilly, France., Gal-Mor O; The Infectious Diseases Research Laboratory, Sheba Medical Center, Tel-Hashomer, Israel.; The Department of Clinical Microbiology and Immunology, Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Grépinet O; INRAE, Université de Tours, ISP, Nouzilly, France., Virlogeux-Payant I; INRAE, Université de Tours, ISP, Nouzilly, France. isabelle.virlogeux-payant@inrae.fr.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2427, pp. 235-248.
DOI:	10.1007/978-1-0716-1971-1_19
Abstrakt:	Applications of bioluminescence for the in vivo study of pathogenic microorganisms are numerous, ranging from the quantification of virulence gene expression to measuring the effect of antimicrobial molecules on the colonization of tissues and organs by the pathogen. Most studies are performed in mice, but recent works demonstrate that this technique is applicable to larger animals like fish, guinea pigs, ferrets, and chickens. Here, we describe the construction and the utilization of a constitutively luminescent strain of Salmonella Typhimurium to monitor in vivo and ex vivo the colonization of mice in the gastroenteritis, typhoid fever, and asymptomatic carriage models of Salmonella infection. (© 2022. The Author(s).)
Databáze:	MEDLINE
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