Expression of CD44 Isoforms in Tumor Samples and Cell Lines of Human Colorectal Cancer.
Autor: | Novosad VO; Faculty of Biology and Biotechnologies, National Research University Higher School of Economics (HSE University), Moscow, Russia. victor.o.novosad@gmail.com., Polikanova IS; Faculty of Biology and Biotechnologies, National Research University Higher School of Economics (HSE University), Moscow, Russia., Tonevitsky EA; Faculty of Biology and Biotechnologies, National Research University Higher School of Economics (HSE University), Moscow, Russia., Maltseva DV; Faculty of Biology and Biotechnologies, National Research University Higher School of Economics (HSE University), Moscow, Russia. |
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Jazyk: | angličtina |
Zdroj: | Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2022 May; Vol. 173 (1), pp. 155-159. Date of Electronic Publication: 2022 May 27. |
DOI: | 10.1007/s10517-022-05512-4 |
Abstrakt: | Detection of colorectal cancer biomarkers (CRC) remains an urgent task for the diagnosis and prediction of the disease course. A promising approach is the study of cancer stem cell markers. The cell surface glycoprotein CD44 is very important for CRC and its stem cells. Alternative splicing of 9 variable exons of CD44 mRNA leads to the formation of various isoforms of the protein with different roles in the progression of cancer. Studies of the functions of CD44 isoforms require adequate models considering the distribution of CD44 isoforms in real tumor samples. In the present study, the expression profile of CD44 isoforms in CRC was assessed based on the publicly available mRNA sequencing data of patient tumors from the TCGA-COAD database. It was shown that normal tissues predominantly expressed isoforms 3 and 4 at nearly equal levels, whereas tumors mainly expressed isoforms 2, 3, and 4; isoform 3 was expressed at the highest level. Further, the most relevant cell lines for studying the role of CD44 in CRC were identified based on the analysis of mRNA sequencing data of 55 CRC cell lines form CCLE database. (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.) |
Databáze: | MEDLINE |
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