Differences in Protein Secretion by Multipotent Mesenchymal Stromal Cells Effective and Ineffective in the Prevention of Acute Graft-Versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation.

Autor: Petinati NA; National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia. loel@mail.ru., Drize NI; National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia., Arapidi GP; National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia., Shender VO; National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia., Lagar'kova MA; National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia., Kuz'mina LA; National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia., Parovichnikova EN; National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia., Savchenko VG; National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2022 May; Vol. 173 (1), pp. 128-132. Date of Electronic Publication: 2022 May 27.
DOI: 10.1007/s10517-022-05507-1
Abstrakt: Multipotent mesenchymal stromal cells (MSC) were administered to patients after allogeneic hematopoietic stem cell transplantation to prevent the development of acute graft-versus- host disease (GVHD). The injection of MSC did not always prevent the development of GVHD. The aim of the work was to compare the secretome of MSC effective and ineffective in the prevention of GVHD. MSC were obtained from the bone marrow of hematopoietic stem cells donors. The secretome was studied using a TripleTOF 5600+ mass spectrometer with a NanoSpray III ion source coupled to a NanoLC Ultra 2D Plus nano-HPLC System. A total of 1,965 proteins were analyzed. Analysis of the secretome of effective and ineffective MSC samples revealed significant differences in the secretion of 1,119 proteins associated with ribosomes, exosomes, focal contacts, and others. Analysis of proteins secreted by MSC can be used to identify prognostically effective samples.
(© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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