Voluntary physical activity mitigates alveolar bone loss in mice with ligature-induced experimental periodontitis.

Autor: Bertolini NO; Veterinary Medicine Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil., Pereira GJS; Health Sciences Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil., Silva VO; Medicine Department, Athens University Center (UNIATENAS), Paracatu, Minas Gerais, Brazil., de Molon RS; Department of Clinical Dentistry, Federal University of Rio de Janeiro, Cidade Universitária, 21941-617 Rio de Janeiro, Brazil., Morari J; Laboratory of Cell Signaling and Obesity and Comorbidities Research Center, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Velloso LA; Laboratory of Cell Signaling and Obesity and Comorbidities Research Center, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Andrade EF; Health Sciences Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil; Institute of Agrarian Sciences, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Unaí, Minas Gerais, Brazil., Pereira LJ; Health Sciences Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil. Electronic address: lucianojosepereira@ufla.br., Moura RF; Health Sciences Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil. Electronic address: rodrigo.moura@ufla.br.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2022 Aug; Vol. 140, pp. 105451. Date of Electronic Publication: 2022 May 11.
DOI: 10.1016/j.archoralbio.2022.105451
Abstrakt: Objective: To investigate the effect of voluntary physical activity (VPA) on inflammatory profile and the progression of experimental periodontal disease (PD) in mice.
Methods: Male C57BL/6 mice were randomly distributed into Control; VPA; PD and PD/VPA groups. We registered VPA (total volume of revolutions) and average speed (revolutions/minute) in a free running wheel for 30 days. On the 15th day, animals from the PD and PD/VPA groups received ligatures on the upper second molars bilaterally. On the 30th day animals were euthanized, and PD progression was assessed by measuring alveolar bone loss (ABL - the linear distance between the cemento-enamel junction and the alveolar bone crest on the teeth buccal surface). Gene expression of RANKL (kappa nuclear factor B receptor) OPG (osteoprotegerin), IL-1β (interleukin 1 beta), IL-6 (interleukin 6) and TNF-α (tumor necrosis factor alpha) were evaluated by real-time PCR (quantitative Polymerase Chain Reaction - relative gene expression).
Results: The total volume of physical activity and the activity speed decreased along the seven days after ligature-placement (p < 0.05), returning to a similar pattern in relation to VPA group. Ligature placement produced significant bone resorption, and increased RANKL, IL-1β, IL-6 and TNF-α expression. VPA reduced ABL (p < 0,05) and the expression of TNF-α and IL-1β, whereas increased OPG expression.
Conclusion: Animals induced to PD with access to the VPA wheel presented both lower gingival inflammation and less alveolar bone resorption in comparison to animals without access to the wheel.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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