Amelioration of aluminum-induced hepatic and nephrotoxicity by Premna odorata extract is mediated by lowering MMP9 and TGF-β gene alterations in Wistar rat.

Autor: Ahmed WMS; Department of Clinical Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt., Ibrahim MA; Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. marwaibrahim@cu.edu.eg., Helmy NA; Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt., ElKashlan AM; Department of Biochemistry, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt., Elmaidomy AH; Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt., Zaki AR; Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2022 Oct; Vol. 29 (48), pp. 72827-72838. Date of Electronic Publication: 2022 May 26.
DOI: 10.1007/s11356-022-20735-8
Abstrakt: This study aims to investigate the effect of Premna odorata (P. odorata) (Lamiaceae) on the hepatic and nephrotoxicity induced by aluminum chloride (AlCl 3 ) in rat. Wistar male rats were equally classified into four groups: control, P. odorata extract (500 mg/kg B.W.), AlCl 3 (70 mg/kg B.W.), and P. odorata extract plus AlCl 3 groups. All treatments were given orally for 4 weeks. Serum transaminases and some biochemical parameters, hepatic and renal antioxidant/oxidant biomarker; tumor necrosis factor-α (TNF-α); matrix metalloproteinase (MMP9) and transforming growth factor-β (TGF-β) mRNA expression; histopathological examination of the liver, and kidneys were investigated. The obtained results revealed that AlCl 3 significantly increased the activities of serum aspartate transaminase, alanine transaminase, and alkaline phosphatase as well as produced a significant increase in total cholesterol, triglyceride, urea, and creatinine concentrations, while there were no changes observed in the total protein, albumin, and globulin concentrations. Also, aluminum administration significantly decreased the reduced glutathione content and increased the catalase activity, malondialdehyde, and TNF-α concentrations in the liver and kidney tissue. Moreover, AlCl 3 results in congestion, degeneration, and inflammation of the liver and kidney tissue. Co-treatment of P. odorata extract with AlCl 3 alleviated its harmful effects on the previous parameters and reduced the histopathological alterations induced by AlCl 3 . Therefore, Premna odorata may have a potent protective effect against oxidative stress induced by Al toxicity through downregulation of MMP9 and TGF-β gene expression.
(© 2022. The Author(s).)
Databáze: MEDLINE
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