Natural component geniposide enhances survival rate of crayfish Procambarus clarkii infected with white spot syndrome virus.

Autor: Huang AG; Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, China; School of Marine Sciences, Guangxi University, Nanning, 530004, China; College of Life Science and Technology, Guangxi University, Nanning, 530004, China., He WH; Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, China; School of Marine Sciences, Guangxi University, Nanning, 530004, China., Zhang FL; Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, China; School of Marine Sciences, Guangxi University, Nanning, 530004, China., Wei CS; Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, China; School of Marine Sciences, Guangxi University, Nanning, 530004, China., Wang YH; Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, China; School of Marine Sciences, Guangxi University, Nanning, 530004, China. Electronic address: wyh@gxu.edu.cn.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2022 Jul; Vol. 126, pp. 96-103. Date of Electronic Publication: 2022 May 22.
DOI: 10.1016/j.fsi.2022.05.037
Abstrakt: White Spot Disease (WSD), caused by white spot syndrome virus (WSSV), is an acute and highly lethal viral disease of shrimp. Currently, there are no commercially available drugs to control WSD. It is urgent and necessary to find anti-WSSV drugs. Natural compounds are an important source of antiviral drug discovery. In this study, the anti-WSSV activity of natural compound geniposide (GP) was investigated in crayfish Procambarus clarkii. Results showed that GP had a concentration-dependent inhibitory effect on WSSV replication in crayfish at 24 h, and highest inhibition was more than 98%. In addition, GP significantly inhibited the expression of WSSV immediate-early gene ie1, early gene DNApol, late gene VP28. The mortality of WSSV-infected crayfish in control groups was 100%, while it reduced by 70.0% when treated with 50 mg/kg GP. Co-incubation, pre-treatment and post-treatment experiments showed that GP could prevent and treat WSSV infection in crayfish by significantly inhibiting WSSV multiplication. Mechanistically, the syntheses of WSSV structural proteins VP19, VP24, VP26 and VP28 were significantly inhibited by GP in S2 cells. Furthermore, GP could also suppress WSSV replication by blocking the expression of antiviral immunity-related factor STAT to reduce ie1 transcription. Moreover, GP possessed anti-inflammatory and anti-oxidative activity in crayfish. Overall, GP has the potential to be developed as a preventive or therapeutic agent against WSSV infection.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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