| Autor: |
Bacelar PAA; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.; Oswaldo Cruz Foundation Regional Office-Piauí, Teresina, Piauí, Brazil., Jaeger LH; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Juiz de Fora Federal University, Juiz de Fora, Brazil., Calegar DA; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Santos JP; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.; Oswaldo Cruz Foundation Regional Office-Piauí, Teresina, Piauí, Brazil., Coronato-Nunes B; Arthur Sá Earp Neto University Center/Petropolis Faculty of Medicine (UNIFASE/FMP), Petrópolis, Rio de Janeiro, Brazil., Reis ERC; Municipal Health Department of Nossa Senhora de Nazaré, Nossa Senhora de Nazaré, Piauí, Brazil., Bóia MN; Laboratory of Biology and Parasitology of Wild Reservoirs Mammals, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Monteiro KJL; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.; Oswaldo Cruz Foundation Regional Office-Piauí, Teresina, Piauí, Brazil., Carvalho-Costa FA; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. |
| Abstrakt: |
Metastrongylosis is an infection of the respiratory tract of pigs caused by parasites of the genus Metastrongylus , whose eggs are similar to other Strongylida through light microscopy; species-specific identification can be performed with molecular tools. We explored the species composition and the genetic diversity of Metastrongylus infecting pigs in close contact with humans in impoverished rural communities in the state of Piauí, in northeastern Brazil. Fecal samples ( n = 78) were collected for parasitologic tests. Egg morphometry and molecular characterization, using the cytochrome c oxidase subunit 1 ( cox 1) gene, were performed. For strongyliform eggs, 62 of 78 (80%) pigs were positive and 6 of 99 (6%) eggs had dimensions compatible with Metastrongylus . Of the 37 samples submitted to PCR, 10 were identified as M. salmi . We found 3 M. salmi haplotypes, including 2 new and 1 described previously in Europe. Overall, M. salmi demonstrated lower intraspecific genetic diversity: diversity index (H) ± SD = 0.318 ± 0.164, n = 12, compared with published M. pudendotectus sequences (1.000 ± 0.272, n = 3). To our knowledge, M. salmi DNA sequences have not been published previously from pigs in South America. |
