Guiding cell migration in 3D with high-resolution photografting.

Autor: Sayer S; Research Group 3D Printing and Biofabrication, Institute of Materials Science and Technology, TU Wien, Vienna, Austria.; Austrian Cluster for Tissue Regeneration (https://www.tissue-regeneration.at), Vienna, Austria., Zandrini T; Research Group 3D Printing and Biofabrication, Institute of Materials Science and Technology, TU Wien, Vienna, Austria.; Austrian Cluster for Tissue Regeneration (https://www.tissue-regeneration.at), Vienna, Austria., Markovic M; Research Group 3D Printing and Biofabrication, Institute of Materials Science and Technology, TU Wien, Vienna, Austria.; Austrian Cluster for Tissue Regeneration (https://www.tissue-regeneration.at), Vienna, Austria., Van Hoorick J; Polymer Chemistry and Biomaterials Group, Centre of Macromolecular Chemistry, Department of Organic and Macromolecular Chemistry, Ghent University, Ghent, Belgium., Van Vlierberghe S; Polymer Chemistry and Biomaterials Group, Centre of Macromolecular Chemistry, Department of Organic and Macromolecular Chemistry, Ghent University, Ghent, Belgium., Baudis S; Austrian Cluster for Tissue Regeneration (https://www.tissue-regeneration.at), Vienna, Austria.; Polymer Chemistry and Technology Group, Institute of Applied Synthetic Chemistry, TU Wien, Vienna, Austria., Holnthoner W; Austrian Cluster for Tissue Regeneration (https://www.tissue-regeneration.at), Vienna, Austria.; Ludwig-Boltzmann-Institute for Traumatology, The Research Centre in Cooperation with AUVA, Vienna, Austria., Ovsianikov A; Research Group 3D Printing and Biofabrication, Institute of Materials Science and Technology, TU Wien, Vienna, Austria. Aleksandr.Ovsianikov@tuwien.ac.at.; Austrian Cluster for Tissue Regeneration (https://www.tissue-regeneration.at), Vienna, Austria. Aleksandr.Ovsianikov@tuwien.ac.at.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 May 23; Vol. 12 (1), pp. 8626. Date of Electronic Publication: 2022 May 23.
DOI: 10.1038/s41598-022-11612-y
Abstrakt: Multi-photon lithography (MPL) has proven to be a suitable tool to precisely control the microenvironment of cells in terms of the biochemical and biophysical properties of the hydrogel matrix. In this work, we present a novel method, based on multi-photon photografting of 4,4'-diazido-2,2'-stilbenedisulfonic acid (DSSA), and its capabilities to induce cell alignment, directional cell migration and endothelial sprouting in a gelatin-based hydrogel matrix. DSSA-photografting allows for the fabrication of complex patterns at a high-resolution and is a biocompatible, universally applicable and straightforward process that is comparably fast. We have demonstrated the preferential orientation of human adipose-derived stem cells (hASCs) in response to a photografted pattern. Co-culture spheroids of hASCs and human umbilical vein endothelial cells (HUVECs) have been utilized to study the directional migration of hASCs into the modified regions. Subsequently, we have highlighted the dependence of endothelial sprouting on the presence of hASCs and demonstrated the potential of photografting to control the direction of the sprouts. MPL-induced DSSA-photografting has been established as a promising method to selectively alter the microenvironment of cells.
(© 2022. The Author(s).)
Databáze: MEDLINE
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