| Autor: |
Simpson DJ; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada., Zhang JS; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada., D'Amico V; University of Natural Resources and Life Sciences Vienna (BOKU), Department of Food Science and Technology, Institute of Food Science, Muthgasse 18, Vienna, 1190, Austria., Llamas-Arriba MG; Departamento de Química Aplicada, Facultad de Químicas, Universidad del País Vasco (UPV/EHU), 20018 San Sebastián, Spain., Gänzle MG; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada. |
| Abstrakt: |
Genomic characterization of Furfurilactobacillus rossiae revealed that strains which were previously identified as F. rossiae are genetically heterogeneous. The 16S rRNA gene sequences of strains FUA3430, FUA3583, C5, FUA3115 and FUA3119, were 99.6 % identical to F. rossiae but the core genome analysis revealed that these strains share less than 93 % average nucleotide identity (ANI) with the F. rossiae type strain DSM 15814 T . Because the ANI value is below the threshold for delineation of bacterial species, we propose the novel species Furfurilactobacillus milii sp. nov. with the type strain FUA3430 T (=DSM 113338 T =LMG 32478 T ). Strains of F. milii have smaller genomes than F. rossiae , lack the pdu-cbi-cob-hem cluster which is responsible for 1,2-propanediol utilization in F. rossiae , and lack genes involved in ethanolamine utilization. Two strains of the novel species (FUA3430 T and FUA3583) were compared to F. rossiae FUA3214. Analysis of the cellular fatty acid composition and metabolite analysis did not reveal significant differences between F. milii sp. nov. and F. rossiae FUA3124. Although the growth requirements with respect to temperature and pH were very similar, only the strain of F. rossiae utilized melibiose and d-xylose. Morphological differences were also seen in the colony and cell size of the novel compared to F. rossiae . |
