Effect of Lactobacillus dominance modified by Korean Red Ginseng on the improvement of Alzheimer's disease in mice.

Autor:	Lee M; Department of Neurology, Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea., Lee SH; Department of Neurology, Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea., Kim MS; Functional Genome Institute, PDXen. Biosystem Co., Gyeongi-do, Republic of Korea., Ahn KS; Functional Genome Institute, PDXen. Biosystem Co., Gyeongi-do, Republic of Korea., Kim M; Department of Neurology, Biomedical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea.; Neuroscience Dementia Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.; Protein Metabolism Medical Research Center, College of Medicine, Seoul National University Hospital, Seoul, Republic of Korea.
Jazyk:	angličtina
Zdroj:	Journal of ginseng research [J Ginseng Res] 2022 May; Vol. 46 (3), pp. 464-472. Date of Electronic Publication: 2021 Nov 11.
DOI:	10.1016/j.jgr.2021.11.001
Abstrakt:	Background: Gut microbiota influence the central nervous system through gut-brain-axis. They also affect the neurological disorders. Gut microbiota differs in patients with Alzheimer's disease (AD), as a potential factor that leads to progression of AD. Oral intake of Korean Red Ginseng (KRG) improves the cognitive functions. Therefore, it can be proposed that KRG affect the microbiota on the gut-brain-axis to the brain. Methods: Tg2576 were used for the experimental model of AD. They were divided into four groups: wild type (n = 6), AD mice (n = 6), AD mice with 30 mg/kg/day (n = 6) or 100 mg/kg/day (n = 6) of KRG. Following two weeks, changes in gut microbiota were analyzed by Illumina HiSeq4000 platform 16S gene sequencing. Microglial activation were evaluated by quantitative Western blot analyses of Iba-1 protein. Claudin-5, occludin, laminin and CD13 assay were conducted for Blood-brain barrier (BBB) integrity. Amyloid beta (Aβ) accumulation demonstrated through Aβ 42/40 ratio was accessed by ELISA, and cognition were monitored by Novel object location test. Results: KRG improved the cognitive behavior of mice (30 mg/kg/day p < 0.05; 100 mg/kg/day p < 0.01), and decreased Aβ 42/40 ratio (p < 0.01) indicating reduced Aβ accumulation. Increased Iba-1 (p < 0.001) for reduced microglial activation, and upregulation of Claudin-5 (p < 0.05) for decreased BBB permeability were shown. In particular, diversity of gut microbiota was altered (30 mg/kg/day q-value<0.05), showing increased population of Lactobacillus species. (30 mg/kg/day 411%; 100 mg/kg/day 1040%). Conclusions: KRG administration showed the Lactobacillus dominance in the gut microbiota. Improvement of AD pathology by KRG can be medicated through gut-brain axis in mice model of AD. Competing Interests: The authors declare that they have no competing interests. (© 2021 The Korean Society of Ginseng. Publishing services by Elsevier B.V.)
Databáze:	MEDLINE
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