Layers 3 and 4 Neurons of the Bilateral Whisker-Barrel Cortex.

Autor: Tsytsarev V; Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore 20 Penn St, HSF-2, 21201 MD, Baltimore, United States. Electronic address: tsytsarev@umaryland.edu., Kwon SE; Department of Neuroscience, John Hopkins School of Medicine, 855 N. Wolfe Street, Rangos 295, Baltimore, MD 21205, United States. Electronic address: skwon21@jhmi.edu., Plachez C; Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore 20 Penn St, HSF-2, 21201 MD, Baltimore, United States. Electronic address: cplachez@som.umaryland.edu., Zhao S; Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore 20 Penn St, HSF-2, 21201 MD, Baltimore, United States. Electronic address: SZhao@som.umaryland.edu., O'Connor DH; Department of Neuroscience and Krieger Mind/Brain Institute Johns Hopkins University, 3400 N Charles St, 338 Krieger Hall, Baltimore, MD 21218, United States. Electronic address: dan.oconnor@jhmi.edu., Erzurumlu RS; Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore 20 Penn St, HSF-2, 21201 MD, Baltimore, United States. Electronic address: RErzurumlu@som.umaryland.edu.
Jazyk: angličtina
Zdroj: Neuroscience [Neuroscience] 2022 Jul 01; Vol. 494, pp. 140-151. Date of Electronic Publication: 2022 May 20.
DOI: 10.1016/j.neuroscience.2022.05.018
Abstrakt: In Robo3 R3-5 cKO mouse brain, rhombomere 3-derived trigeminal principal nucleus (PrV) neurons project bilaterally to the somatosensory thalamus. As a consequence, whisker-specific neural modules (barreloids and barrels) representing whiskers on both sides of the face develop in the sensory thalamus and the primary somatosensory cortex. We examined the morphological complexity of layer 4 barrel cells, their postsynaptic partners in layer 3, and functional specificity of layer 3 pyramidal cells. Layer 4 spiny stellate cells form much smaller barrels and their dendritic fields are more focalized and less complex compared to controls, while layer 3 pyramidal cells did not show notable differences. Using in vivo 2-photon imaging of a genetically encoded fluorescent [Ca 2+ ] sensor, we visualized neural activity in the normal and Robo3 R3-5 cKO barrel cortex in response to ipsi- and contralateral single whisker stimulation. Layer 3 neurons in control animals responded only to their contralateral whiskers, while in the mutant cortex layer 3 pyramidal neurons showed both ipsi- and contralateral whisker responses. These results indicate that bilateral whisker map inputs stimulate different but neighboring groups of layer 3 neurons which normally relay contralateral whisker-specific information to other cortical areas.
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Databáze: MEDLINE
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