[Induced biosynthesis of fengycin and surfactin in a strain of Bacillus amyloliquefaciens with oomyceticidal activity on zoospores of Phytophthora capsici].

Autor:	Ley-López N; Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación Culiacán (CIAD), Culiacán, Sinaloa, México., Basilio Heredia J; Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación Culiacán (CIAD), Culiacán, Sinaloa, México., San Martín-Hernández C; Colegio de Postgraduados, Campus Montecillo, Texcoco, Estado de México, México., Ibarra-Rodríguez JR; Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación Culiacán (CIAD), Culiacán, Sinaloa, México., Angulo-Escalante MÁ; Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación Culiacán (CIAD), Culiacán, Sinaloa, México., García-Estrada RS; Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación Culiacán (CIAD), Culiacán, Sinaloa, México. Electronic address: rsgarcia@ciad.mx.
Jazyk:	Spanish; Castilian
Zdroj:	Revista Argentina de microbiologia [Rev Argent Microbiol] 2022 Jul-Sep; Vol. 54 (3), pp. 181-191. Date of Electronic Publication: 2022 May 18.
DOI:	10.1016/j.ram.2022.03.002
Abstrakt:	A potential alternative to the use of chemical products with oomyceticidal action for the control of Phytophthora capsici in vegetables is the use of antimicrobial metabolites, biosynthesized in Bacillus species. The objective of this study was to induce the biosynthesis of lipopeptides in Bacillus amyloliquefaciens KX953161.1 by using glutamic acid, iron, cellulose, chitin, or inactive Colletotrichum spp. cells. The in vitro oomyceticidal effect of the bacterial lipopeptides on zoospores of Phytophthora capsici was evaluated. The lipopeptides identified and quantified in the crude extracts by high performance thin layer chromatography (HPTLC) were fengycin and surfactin. The bacterial culture with inactive fungal cells yielded the greatest biosynthesis of lipopeptides, at 1847.02± 11.8 and 2563.45± 18.4 μg/ml of fengycin and surfactin, respectively and the treatments that obtained lower production of these lipopeptides, were those to which iron and cellulose were added with 608.05 ± 22.6 and 903.74± 22.1; 563.31± 11.9 and 936.96± 41.1 μg/ml for fengicin and surfactin, respectively. The lipopeptide extracted showed 100% germination inhibition on zoospores of P. capsici, revealing encystment, malformations in the germ tube and cellular degradation. Lipopeptides have the potential to control P. capsici; however, the biosynthesis of these lipopeptides requires further study to determine their biological mode of action and optimize lipopeptide performance and profile. (Copyright © 2022 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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