Proteomic analysis of F 1 hybrids and intermediate variants in a Littorina saxatilis hybrid zone.

Autor: Diz AP; Centro de Investigación Mariña (CIM-UVIGO), Universidade de Vigo, Vigo 36310, Spain., Romero MR; Centro de Investigación Mariña (CIM-UVIGO), Universidade de Vigo, Vigo 36310, Spain., Galindo J; Centro de Investigación Mariña (CIM-UVIGO), Universidade de Vigo, Vigo 36310, Spain., Saura M; Departamento de Mejora Genética Animal, INIA, Madrid 28040, Spain., Skibinski DOF; Institute of Life Science, Swansea University Medical School, Swansea SA2 8PP, UK., Rolán-Alvarez E; Centro de Investigación Mariña (CIM-UVIGO), Universidade de Vigo, Vigo 36310, Spain.
Jazyk: angličtina
Zdroj: Current zoology [Curr Zool] 2021 Jul 10; Vol. 68 (3), pp. 351-359. Date of Electronic Publication: 2021 Jul 10 (Print Publication: 2022).
DOI: 10.1093/cz/zoab054
Abstrakt: Proteomic analysis was carried out on the Crab (upper-shore) and Wave (lower-shore) ecotypes of Littorina saxatilis from a hybrid zone at Silleiro Cape, Spain. Proteome profiles of individual snails were obtained. Protein expression in F 1 hybrid snails bred in the laboratory and snails with intermediate shell phenotypes collected from the mid-shore were compared with Crab and Wave ecotypes using analytical approaches used to study dominance. Multivariate analysis over many protein spots showed that the F 1 snails are distinct from both ecotypes but closer to the Wave ecotype. The intermediate snails are highly variable, some closer to the Crab and others to the Wave ecotype. Considered on a protein by protein basis, some proteins are significantly closer in expression to the Crab and others to the Wave ecotype for both F 1 and intermediate snails. Furthermore, a significant majority of proteins were closer in expression to the Wave ecotype for the F 1 , consistent with the multivariate analysis. No such significant majority toward either the Crab or Wave ecotype was observed for the intermediate snails. The closer similarity of F 1 and Wave ecotype expression patterns could be the result of similar selective pressures in the similar mid-shore and low-shore environments. For a significantly larger number of proteins, intermediate snails were closer in expression to the ecotype having the lower expression, for both Crab and Wave ecotypes. This is somewhat unexpected as lower expression might be expected to be an indication of impairment of function and lower fitness. Proteomic analysis could be important for the identification of candidate proteins useful for gaining improved understanding of adaptation and barriers to gene flow in hybrid zones.
(© The Author(s) (2021). Published by Oxford University Press on behalf of Editorial Office, Current Zoology.)
Databáze: MEDLINE
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