Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity.

Autor:	Guizzo MG; Vector Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Rockville, MD, United States.; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Tirloni L; Tick-Pathogen Transmission Unit, Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT, United States., Gonzalez SA; Instituto de Agrobiotecnologia y Biologia Molecular (IABIMO), INTA-CONICET, Hurlingham, Argentina., Farber MD; Instituto de Agrobiotecnologia y Biologia Molecular (IABIMO), INTA-CONICET, Hurlingham, Argentina., Braz G; Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Parizi LF; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Dedavid E Silva LA; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., da Silva Vaz I Jr; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.; Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Rio de Janeiro, Brazil., Oliveira PL; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Rio de Janeiro, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2022 May 03; Vol. 13, pp. 868575. Date of Electronic Publication: 2022 May 03 (Print Publication: 2022).
DOI:	10.3389/fmicb.2022.868575
Abstrakt:	In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Guizzo, Tirloni, Gonzalez, Farber, Braz, Parizi, Dedavid e Silva, da Silva Vaz and Oliveira.)
Databáze:	MEDLINE
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