Ethacrynic acid and cinnamic acid combination exhibits selective anticancer effects on K562 chronic myeloid leukemia cells.

Autor: Yenigül M; Graduate School of Engineering and Science, Bioengineering Department, Abdullah Gul University, Kayseri, Turkey., Akçok İ; Faculty of Life and Natural Sciences, Bioengineering Department, Abdullah Gul University, Kayseri, Turkey., Gencer Akçok EB; Faculty of Life and Natural Sciences, Molecular Biology and Genetics Department, Abdullah Gul University, 38080, Kayseri, Turkey. emelbasak.gencerakcok@agu.edu.tr.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2022 Aug; Vol. 49 (8), pp. 7521-7530. Date of Electronic Publication: 2022 May 18.
DOI: 10.1007/s11033-022-07560-5
Abstrakt: Background: Despite the recent advances in chemotherapy, the outcomes and the success of these treatments still remain insufficient. Novel combination treatments and treatment strategies need to be developed in order to achieve more effective treatment. This study was designed to investigate the combined effect of ethacrynic acid and cinnamic acid on cancer cell lines.
Methods: The anti-proliferative effect of ethacrynic acid and cinnamic acid was investigated by MTT cell viability assay in three different cancer cell lines. Combination indexes were calculated using CompuSyn software. Apoptosis was assessed by flow cytometric Annexin V-FITC/PI double-staining. The effect of the inhibitors on cell cycle distribution was measured by propidium iodide staining.
Results: The combination treatment of ethacrynic acid and cinnamic acid decreased cell proliferation significantly, by 63%, 75% and 70% for K562, HepG2 and TFK-1 cells, respectively. A 5.5-fold increase in the apoptotic cell population was observed after combination treatment of K562 cells. The population of apoptotic cells increased by 9.3 and 0.4% in HepG2 and TFK-1 cells, respectively. Furthermore, cell cycle analysis shows significant cell cycle arrest in S and G2/M phase for K562 cells and non-significant accumulation in G0/G1 phase for TFK-1 and HepG2 cells.
Conclusions: Although there is a need for further investigation, our results suggest that the inhibitors used in this study cause a decrease in cellular proliferation, induce apoptosis and cause cell cycle arrest.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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