IMMUNE RESPONSE UPON THE ADMINISTRATION OF RECOMBINANT PROTEIN ANTIBODIES Ag-38 KDa Mycobacterium tuberculosis AND RIFAMPICIN EX-VIVO .

Autor:	Raras TYM; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Brawijaya Malang. Indonesia., Fahrinda A; Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya. Malang Indonesia., Yuliati; Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya. Malang Indonesia., Nurhidayati DY; Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya. Malang Indonesia., Sujuti H; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Brawijaya Malang. Indonesia., Prawiro SR; Department of Clinical Microbiology, Faculty of Medicine, Universitas Brawijaya. Malang Indonesia.
Jazyk:	angličtina
Zdroj:	African journal of infectious diseases [Afr J Infect Dis] 2022 May 06; Vol. 16 (2), pp. 71-79. Date of Electronic Publication: 2022 May 06 (Print Publication: 2022).
DOI:	10.21010/Ajid.v16i2.8
Abstrakt:	Background: Development a granuloma model resembling latent tuberculosis in vitro is needed with a fast and efficient time to be used as an effective therapy. This study aimed to form efficient granulomas, increase cellular immunity and humoral immunity, and evaluate growth on media using recombinant protein antibody Ag38kDa, Rifampicin, and a combination of both. Peripheral Blood Mononuclear Cell (PBMC) in vitro is derived from a healthy individual separated from monocytes and lymphocytes. Materials and Methods: Monocytes are matured into macrophages and then combined macrophages and lymphocytes to the Roswell Park Memorial Institute (RPMI) medium. Flow cytometry analysis was used to count the number of cells, and cytokine levels were measured using ELISA. The result from the treatment was planted on the Lowenstein-Jensen medium. Results: Granulomas-like aggregates was formed after one-day post-infection with Mycobacterium tuberculosis ( M.tb ). A significant increase in immune response occurred in the number of macrophages, Th1, and Tregs in the combination group compared to the Mtb infection group. The number of Th2 and Th17 cells in the combination group was compared with the control but not significantly. TNF-α cytokine levels increased in the combination group compared to Mtb infection, while in IL-4, we found between all groups, there was no significant difference. Bacterial colonies on culture in the Lowenstein-Jensen medium were only seen in positive controls. Conclusion: Our study concluded that administration of a combination between Ag38kDa recombinant antibody and rifampicin could inhibit granuloma formation and enhance immune response. (Copyright: © 2022 Afr. J. Infect. Diseases.)
Databáze:	MEDLINE
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