Expression of proliferation-related genes in BM-MSC-treated ALL cells in hypoxia condition is regulated under the influence of epigenetic factors in-vitro.

Autor: Yang X; Department of Hematology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou City, 450052, Henan Province, China., Wang Y; Department of Hematology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou City, 450052, Henan Province, China., Rahman HS; Department of Physiology, College of Medicine, University of Sulaimani, Sulaimaniyah, Iraq., Mohammad TAM; Department of Pharmacology and Toxicology, College of Pharmacy, HMU (Hawler Medical University), Erbil, Iraq., Sorkhabi AD; Medical Laboratory Analysis Department, College of Health Sciences, Cihlan University of Sulaimaniya, Kurdistan Region, Iraq.; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Korsakov SE; Department of maxillofacial surgery, I. M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Thangavelu L; Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Saveetha University, Chennai, India., Adili A; Department of Oncology, Tabriz University of Medical Sciences, Tabriz, Iran.; Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Sarkesh A; Medical Laboratory Analysis Department, College of Health Sciences, Cihlan University of Sulaimaniya, Kurdistan Region, Iraq.; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Tamjidifar R; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Saeedi H; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Aslaminabad R; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Tarzi S; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Akbari M; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. akbarimo@tbzmed.ac.ir.
Jazyk: angličtina
Zdroj: Medical oncology (Northwood, London, England) [Med Oncol] 2022 May 18; Vol. 39 (5), pp. 88. Date of Electronic Publication: 2022 May 18.
DOI: 10.1007/s12032-022-01671-6
Abstrakt: Mesenchymal stem cells affect ALL cell biology under hypoxic conditions. We studied survival, proliferation, expression, and promoter methylation levels of essential genes involved in expanding MOLT-4 cells co-cultured with BM-MSC under the hypoxic condition. Here, MOLT-4 cells were co-cultured with BMMSCs under hypoxic conditions. First, the apoptosis rate was evaluated by Flow cytometry. Then, MOLT-4 cells' proliferation rate was assessed using MTT assay, and the expressions and methylation rates of genes were determined by qRT-PCR and MS-qPCR, respectively. The results showed that although MOLT-4 cells proliferation and survival rates were reduced under hypoxic conditions, this reduction was not statistically significant. Also, we showed that hypoxic conditions caused upregulation of candidate genes and affected their methylation status. Besides, it was revealed that Pontin was downregulated, while KDM3A, SKP2, and AURKA had an upward trend in the presence of MOLT-4 cells plus BM-MSC. The co-culture of leukemia cells with BMMSCs under hypoxic conditions may be a potential therapeutic approach for ALL.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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