PTH 1-34-functionalized bioactive glass improves peri-implant bone repair in orchiectomized rats: Microscale and ultrastructural evaluation.

Autor: Gomes-Ferreira PHS; Department of Surgery and Integrated Clinic, São Paulo State University, Araçatuba Dental School, Araçatuba, SP, Brazil. Electronic address: pedroferreirabmf@gmail.com., Micheletti C; Department of Materials Science and Engineering, McMaster University, Hamilton, ON, Canada., Frigério PB; São Paulo State University, Araçatuba Dental School, Araçatuba, SP, Brazil., de Souza Batista FR; Department of Surgery and Integrated Clinic, São Paulo State University, Araçatuba Dental School, Araçatuba, SP, Brazil., Monteiro NG; São Paulo State University, Araçatuba Dental School, Araçatuba, SP, Brazil., Bim-Júnior O; Department of Physics, São Paulo State University, School of Sciences, Bauru, SP, Brazil., Lisboa-Filho PN; Department of Physics, São Paulo State University, School of Sciences, Bauru, SP, Brazil., Grandfield K; Department of Materials Science and Engineering, McMaster University, Hamilton, ON, Canada; School of Biomedical Engineering, McMaster University, Hamilton, ON, Canada., Okamoto R; São Paulo State University, Araçatuba Dental School, Araçatuba, SP, Brazil.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2022 Mar; Vol. 134, pp. 112688. Date of Electronic Publication: 2022 Jan 31.
DOI: 10.1016/j.msec.2022.112688
Abstrakt: The objective of this work was to investigate the use of Biogran® functionalized with parathyroid hormone (PTH) 1-34 by sonochemistry for the local delivery of this anabolic agent to the implant site. The effects of Biogran® and topical administration of PTH 1-34 on peri-implant bone regeneration were evaluated from the microscale to ultrastructural levels in healthy (SHAM) and orchiectomized (ORQ). While some animals only received a titanium implant in their tibial metaphyses (CLOT group), in others the peri-implant defect was first filled with Biogran® either without or with PTH 1-34 functionalization (BG and BGPTH groups, respectively) prior to implant installation. Osseointegration was characterized from a biomechanical perspective by measuring the removal torque with the counter-torque technique. Micro-CT was used to evaluate the percentage of bone volume, trabecular thickness, number and separation, and bone-implant contact (BIC). Dynamics of new bone formation were assessed by measuring fluorochrome area, daily mineral apposition rate, and neoformed bone area using confocal laser microscopy. RT-PCR was performed to evaluate ALP and osteocalcin expression. The interface between newly formed bone and Biogran® was examined using scanning electron microscopy (SEM) and scanning transmission electron microscopy (STEM) at the micro-and nanoscale, respectively, while elemental analyses were completed in SEM with energy-dispersive X-ray spectroscopy (EDS). STEM imaging demonstrated the intimate attachment of bone to Biogran® (nanoscale level). Overall, the results suggest that the effectiveness of the topical administration of PTH 1-34 at the implant site seems enhanced in osteoporotic bone, promoting peri-implant bone regeneration to comparable levels in healthy conditions.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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