Increased microchimerism in peripheral blood of women with systemic lupus erythematosus: relation with pregnancy.

Autor:	Bos EMJ; Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands. e.m.j.bos@lumc.nl., Rijnink EC; Department of Internal Medicine, Haaglanden Medical Centre, The Hague, The Netherlands., Zandbergen M; Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands., Diaz de Pool JDN; Department of Obstetrics, Leiden University Medical Center, Leiden, The Netherlands., Almekinders MM; Department of Pathology, Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands., Berden JHM; Department of Nephrology, Radboud University Medical Center, Nijmegen, The Netherlands., Bijl M; Department of Internal Medicine and Rheumatology, Martini Hospital, Groningen, The Netherlands., Hagen EC; Department of Nephrology, Meander Medical Center, Amersfoort, The Netherlands., Kolster-Bijdevaate C; Department of Obstetrics, Leiden University Medical Center, Leiden, The Netherlands., Steup-Beekman GM; Department of Rheumatology, Leiden University Medical Center, Leiden, and Haaglanden Medical Center Bronovo, The Hague, The Netherlands., Wolterbeek R; Department of Medical Statistics, Leiden University Medical Center, Leiden, The Netherlands., Bloemenkamp KWM; Department of Obstetrics, Birth Centre Wilhelmina's Children Hospital, Division Woman and Baby, University Medical Centre Utrecht, University of Utrecht, The Netherlands., Baelde HJ; Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands., Bruijn JA; Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands., Bajema IM; Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands., Wilhelmus S; Department of Pathology, Leiden University Medical Center, Leiden, and Pathan B.V., Laboratory for Pathology, Rotterdam, The Netherlands.
Jazyk:	angličtina
Zdroj:	Clinical and experimental rheumatology [Clin Exp Rheumatol] 2022 Nov; Vol. 40 (11), pp. 2153-2160. Date of Electronic Publication: 2022 Apr 29.
DOI:	10.55563/clinexprheumatol/75tlgf
Abstrakt:	Objectives: We aimed to determine the presence, amount and origin of microchimerism in peripheral blood of pregnant and non-pregnant parous women with systemic lupus erythematosus (SLE) as compared to control subjects. Methods: We performed a comparative study in which peripheral blood was drawn from eleven female non-pregnant SLE-patients and 22 control subjects, and from six pregnant SLE-patients and eleven control subjects during gestation and up to six months postpartum. Quantitative PCR for insertion-deletion polymorphisms and null alleles was used to detect microchimerism in peripheral blood mononuclear cells and granulocytes. Results: Microchimerism was detected more often in non-pregnant SLE-patients than control subjects (54.4% vs. 13.6%, respectively; p=0.03). When present, the median total number of foetal chimeric cells was 5 gEq/106 in patients and 2.5gEq/106 in control subjects (p=0.048). Microchimerism was mostly foetal in origin; maternal microchimerism was detected in one patient and one control subject. In control subjects, microchimerism was always derived from only one source whereas in 50% of patients it originated from multiple sources. The pregnant patients had a significantly higher median number of foetal chimeric cells in the granulocyte fraction just after delivery than control subjects (7.5 gEq/106 vs. 0 gEq/106, respectively; p=0.02). Conclusions: Just after delivery, SLE-patients had more microchimerism than control subjects. Three months post-partum, microchimerism was no longer detectable, only to reappear many years after the last pregnancy, more often and at higher levels in SLE-patients than in control subjects. This suggests that these chimeric cells may originate from non-circulating foetal chimeric stem cells.
Databáze:	MEDLINE
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