Expression profile of synaptic vesicle glycoprotein 2A, B, and C paralogues in temporal neocortex tissue from patients with temporal lobe epilepsy (TLE).

Autor: Pazarlar BA; Neurobiology Research Unit 8057, Neurological Clinic, Copenhagen University Hospital, Rigshospitalet, 6-8 Inge Lehmanns Vej, 2100, Copenhagen, Denmark.; Physiology Department, Faculty of Medicine, Izmir Katip Celebi University, Izmir, Turkey., Aripaka SS; Neurobiology Research Unit 8057, Neurological Clinic, Copenhagen University Hospital, Rigshospitalet, 6-8 Inge Lehmanns Vej, 2100, Copenhagen, Denmark., Petukhov V; Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Pinborg L; Neurobiology Research Unit 8057, Neurological Clinic, Copenhagen University Hospital, Rigshospitalet, 6-8 Inge Lehmanns Vej, 2100, Copenhagen, Denmark.; Department of Neurology, Epilepsy Clinic, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Khodosevich K; Biotech Research and Innovation Centre, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark., Mikkelsen JD; Neurobiology Research Unit 8057, Neurological Clinic, Copenhagen University Hospital, Rigshospitalet, 6-8 Inge Lehmanns Vej, 2100, Copenhagen, Denmark. jens_mikkelsen@dadlnet.dk.; Department of Neuroscience, University of Copenhagen, Copenhagen, Denmark. jens_mikkelsen@dadlnet.dk.
Jazyk: angličtina
Zdroj: Molecular brain [Mol Brain] 2022 May 16; Vol. 15 (1), pp. 45. Date of Electronic Publication: 2022 May 16.
DOI: 10.1186/s13041-022-00931-w
Abstrakt: Synaptic vesicle glycoprotein-2 (SV2) is a family of proteins consisting of SV2A, SV2B, and SV2C. This protein family has attracted attention in recent years after SV2A was shown to be an epileptic drug target and a perhaps a biomarker of synaptic density. So far, the anatomical localization of these proteins in the rodent and human brain have been reported, but co-expression of SV2 genes on a cellular level, their expressions in the human brain, comparison to radioligand binding, any possible regulation in epilepsy are not known. We have here analyzed the expression of SV2 genes in neuronal subtypes in the temporal neocortex in selected specimens by using single nucleus-RNA sequencing, and performed quantitative PCR in populations of temporal lobe epilepsy (TLE) patients and healthy controls. [ 3 H]-UCB-J autoradiography was performed to analyze the correlation between the mRNA transcript and binding capacity to SV2A. Our data showed that the SV2A transcript is expressed in all glutamatergic and GABAergic cortical subtypes, while SV2B expression is restricted to only the glutamatergic neurons and SV2C has very limited expression in a small subgroup of GABAergic interneurons. The level of [ 3 H]-UCB-J binding and the concentration of SV2A mRNA is strongly correlated in each patient, and the expression is lower in the TLE patients. There is no relationship between SV2A expression and age, sex, seizure frequency, duration of epilepsy, or whether patients were recently treated with levetiracetam or not. Collectively, these findings point out a neuronal subtype-specific distribution of the expression of the three SV2 genes, and the lower levels of both radioligand binding and expression further emphasize the significance of these proteins in this disease.
(© 2022. The Author(s).)
Databáze: MEDLINE
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